Fakulti Agroteknologi dan Sains Makanan

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Antioxidant properties, mineral content and sensory acceptance of napier grass teas as affected by different processing methods
(Universiti Malaysia Terengganu, 2020) Ng Khai Shin
Investigation on the configuration of a towline model incorporated with ship towing system performance is obviously necessary to attain navigational towing safety at sea. Instead of employing typical towing models such as single and symmetrical bridle towline models, an asymmetrical bridle towline configuration is then proposed to improve the ship towing performance during operation. This thesis presents Computational Fluid Dynamics (CFD) analysis on the asymmetrical bridle towline model with respect to its effect on course stability and seakeeping performance of the ship towing system in calm water and waves, respectively. Several towing parameters such as various towing angles, towing speeds and towline lengths have been taken into accounts in the simulation. Here, the course stability performance of the towed barge has been analysed in three degrees-of-freedom i.e., surge sway and yaw motions. Meanwhile, the seakeeping characteristic has been assessed at various wavelengths and wave directions involving five degrees-of-freedom i.e., surge, sway, yaw, heave and pitch motions.
Effects of partially cooked frozen 'satar' on the chemical analyses, microbiological quality and sensory acceptance
(Universiti Malaysia Terengganu, 2012) Suriani Ahmad
'Satar' is a popular ready-to-eat food which is grilled before being served. Left over 'Satar' is frequently subjected to frozen and re-grilling. Storing the 'Satar' under freezing condition has been common practice and it will affect the quality of this product. This product may undergo undesirable changes during storage and such deterioration may affect the quality of 'Satar'. The changes of partially cooked frozen 'Satar' during storage and re-grilling have never been studied. Initially, 'Satar' was prepared under controlled environment by mixing the fish, onion, shallot, spices, sugar, salt and shredded coconut together. The chemical analysis was conducted every two weeks storage at -l 8 ° C. The moisture, carbohydrate, protein, lipid and ash contents of the 'Satar' were 66.89%, 5.39%, 11.71 %, 14.06% and 1.87%, respectively. In the present study, the peroxide value of 'Satar' significantly increased from 9.23 to 12.75 mEq/kg fat during frozen storage.
The effects of mixed loading of banana with mango during post harvest storage under ambient temperature
(Universiti Malaysia Terengganu, 2009) Noor Anizah Maarof
Banana which is a climacteric fruit has unique characteristics. Unripe bananas showed a constant low level of ethylene production. Therefore, bananas need to be treated to triggers their ethylene production as example via treatment of mixed loading with mango. The aim of this study is to see the effects of ethylene produce by mango on bananas fruit during storage under ambient temperature. In this study, 1kg of banana were packed together with mango in 1 :0, 1: 1, 1 :2, and 1 :3 ratio by weight Evaluation of ripening of banana based on the surface color change, loss of firmness and the total soluble solid (TSS) contain in banana were taken daily for seven days. For surface color change, treatment of banana: mango (1:3) gave the higher impact on degreening of chlorophyll in banana during second days of storage because it showed a positive a* value compared to the other treatments and control. On the other hand, for loss of firmness treatment of banana: mango (1:2) and treatment of banana: mango (1 :3) had the low firmness value day to day through the time of storage. For the last parameter which is total soluble solid contain treatment of banana: mango (1 :3) had the higher brix value compared to the other treatments on the second day of storage because starch started to convert into sugar. These indicate that treatment of banana: mango (1 :3) had faster ripening rate compared to the others. In the conclusion, treatment of banana: mango with (1:3) ratio by weight is the best ratio to fasten the ripening process for bananas fruit which is until two days earlier if compared with control and I day earlier compared to the other treatments.
Bacteriological study in association with antibiotic and heavy metal resistance pattern of water sample from freshwater giant prawn hatchery
(Terengganu: Universiti Malaysia Terengganu, 2009) Khabibah Md Amin
The total bacterial flora, antibiotic resistance and metal resistance the diseases associated with water sample of giant freshwater prawns post larvae, Macrobrachium rosenbergii were carried out. A study was undertaken to investigate the presence and the total plate count of bacteria isolates by using non selective agar medium such as Trypticase Soy Agar (TSA) and selective agar such as Thiosulfate Citrate Bile Sucrose Agar (TCBS), Pseudomonas Aeromonas Selective Agar Base (GSP), Mac Conkey Agar, Eosin Methylene Blue Agar (EMB) and Xylose Lysine Deoxycholate Agar (XLD). The total bacterial load on TSA, Mac Conkey and GSP agar varied on 102 cfu ml--i with higher counts seen in GSP agar that was 6.1 x 102 bacteria per ml. But the total bacteriological analysis on EMB, TCBS, and XLD agar to few to determine the count. 29 bacterial isolates were identified as Vibrio spp, Escherichia coli, Pseudomonas spp, Salmonella and Aeromonas spp. Heavy metal test with different level concentration such as cadmium dichloride anhydrous (cd) (25, 5.0,10,20 and 40mg/L), cuprum (Cu) (1.5, 300, 600, 1200 and 2400mg/L), Mercuric dichloride (Hg) (2.5, 5, 10, 20, 40μg) and potassium dichromate (Cr) (25, 50, 100, 200 and 400mg/l). The results showed toxicities in the order of Cd=Hg=Cr>Cu. In this pilot study, all bacterial isolates were examined for their resistance to different 15 antibiotics. These antibiotic included: Flumequine (50μg), Nitrofurantoin (50μg), Florfenicol(30μg), Amoxycillin(25μg), Doxycycline (30μg), Oleandomycin (15μg), Tetracycline (30μg), Ampicillin ( lOμg), Lincomycin (15μg), Colistin Sulphate (25μg), Oxolinic Acid (2μg), Novobiocin (30μg), Spiramycin ( lOOμg), Erythromycin (15μg) And Fosfomycin (50μg). Bacterial isolates were indicated that hundred percent resistance to Doxycycline, Oleandomycin, Linomycin, Novobiocin, Spiramycin, Fosfomycin and Flumequine. The lowest incidence of resistance was found in Nitrofurantoin, Florfenicol, Amoxicillin, Tetracyclyin, Ampicillin, Colistin Sulphate, Oxolinic Acid, and Erythromycin. MAR index shown that >0.2 for isolate bacteria indicated that environment was more polluted. Knowledge of the qualitative and quantitative aspects of bacterial flora in the hatchery would help to understand disturbances, if any, brought about during disease outbreaks to M rosenbergii.
Pembangunan kewangan dan pertumbuhan ekonomi di Malaysia
(Universiti Malaysia Terengganu, 2009) Mohd Sobree Mohamed Salleh
Kajian ini bertujuan untuk mengkaji peranan dan kesan pembangunan kewangan kepada pertumbuhan ekonomi di Negara Malaysia. Dalam kajian ini pembolehubah bersandar adalah KDNK dan pembolehubah tidak bersandar adalah Ml, FDI, Kadar Faedah dan Kadar Inflasi. Data yang digunakan adalah sebanyak 30 tahun di mana dari tahun 1975 sehingga tahun 2005. Antara keputusan ialah hanya Ml dan kadar faedah sahaja yang mempengaruhi KDNK Malaysia dalam jangka pendek dan kesemua pembolehubah mempengaruhi KDNK dalam jangka masa panjang.
Fish habitat of aquaculture area in Gong Batu, Setiu Wetland, Terengganu
(Universiti Malaysia Terengganu, 2007) Mohammad Yazid Mohd Sani
A study characteristic of fish habitat was carried out in Setiu Wetland, Setiu, Terengganu on September 2006, November 2006 and February 2007. This study aims to determine the physical, biological and chemical characteristics in specific habitat. For known the water quality parameters, the in-situ apparatus were used and for fish sampling, the gills net were used. From result that have been got, there have twelve species of fishes were found. Mainly then Leiognathus splendens (Kekek) and Glossogobius aureus (Belukor) in studies area. Water quality is in suitable range for fish habitat and aquaculture activities. The temperature ranged from ° 30.45 C to 32.6 ° 1 C, D.O (2.65 mgL- to 13.91 mgL- 1 ), salinity (17.1 ppt to 25.55 ppt), pH (6.795 to 7.385), depth (0.6 m to 1.65 m), turbidity (2.41 to 3.93) and current (0.01 ms-' to 0.155 ms-'). For while monsoon season, the temperature is ranged from 29.45 ° C to 32.85 ° C, 0.0 (2.735 mgL- 1 to 4.46 mgL1 ), salinity (6.20 ppt to 17.30 ppt), pH (5.365 to 10.33), depth (1.3 m to 1.7 m), turbidity (2.30 to 4.13) and current (0.117 ms- 1 to 0.243 ms-').
Effect of substituition of soy milk and coconut milk on physicochemical characteristics and sensory preference of malay chicken curry
(Universiti Malaysia Terengganu, 2012) Yah Xin Yi
Malay chicken curry is a famous cuisine in Malaysia. It is usually meat cooked with combination of spices and coconut milk. Coconut milk is white opaque aqueous extract of coconut that is high in saturated fat. Thus, High intake of Malay chicken curry may lead to an increased level of Low Density Lipoprotein (LDL), the main risk for cardiovascular diseases. This study was carried out to determine physicochemical properties and sensory preference of Malay chicken curry obtained through substitution of coconut milk with soy milk, as one of the ingredients that contains high nutritional values and low in saturated fat, but has not been fully utilized in traditional cuisine. Substitution of coconut milk with soy milk in Malay chicken curry prepared according to the ratio of coconut milk to soy milk (100:0, 75:25, 50:50, 25:75 and 0: 1 OO)was carried out. Results showed substitution of coconut milk with soy milk in Malay chicken curry had significantly (p<0.05) lower its fat and calorie content compared to control of Malay chicken curry. Apart from that, fatty acid profile showed that with higher level of substitution, the lower amount of saturated fat could be found in Malay chicken curry.
Effects of different texture enhancers on the quality and sensory acceptability of roselle pickles
(Universiti Malaysia Terengganu, 2012) Siti Nuraini Sulong
Roselle (Hibiscus sabdari.ffa L) is a species of Hibiscus used for the production of roselle pickles with combination of four types of food grade texture enhancers ( calcium lactate, calcium phosphate, calcium chloride and calcium propionate). Each types of calcium used has different characteristics towards the texture of roselle pickles. This study was conducted to investigate the sensory acceptability, microbiological quality, nutritional content and physicochemical characteristics of roselle pickles using four different types of calcium. The preliminary studies were carried out at three different concentrations (0.5%, 1.0%, and 1.5%) using four types of calcium. The best concentration for calcium lactate and phosphate was 1.0% while for calcium chloride and calcium propionate was 0.5%. Calcium chloride showed the crunchiest texture followed by calcium phosphate, calcium lactate and the least crunchiest was calcium propionate. For sensory acceptance, calcium chloride had received the highest score for most of sensory attributes followed by calcium lactate, calcium phosphate and calcium propionate. For microbiological analyses, there was significant differences (p <0.05) of Aerobic Plate Count (APC) between samples.
The study of prevalence and mean intensity of endoparasite (digenea) on thunnus obesus (bigeye tuna) from South China Sea
(Terengganu: Universiti Malaysia Terengganu, 2009) Nur Aina Lyana Mohamad Ali
Digenean parasite is one of the parasites that affect marine fishes which cause the fish to be lower in quality and unappealing to eat if the number of parasites is high. Study the parasites on fish can contribute to many aspect includes aquatic pollutants and the migration of fish itself. However, the study on digenea from Tuna in Malaysia is rare. So that I choose this project because tuna is one of fisheries sources in our country. In this study, the prevalence and mean intensity of digenea in bigeye tuna become major aspects. 32 Thunnus obesus obtained from South China Sea was dissected to examine digenean parasite from their internal organ. The heart, lymph, pyloric caeca, stomach and intestine was examined under dissecting and compound microscope. All the digenean found was collected and preserved in 70% alcohol in the bijou bottle. The digenea then was stained and drawn under a camera lucida attached to a compound microscope in order to identify the taxonomy of those digenea. The identification was referred to a book written by Yamaguti (1958). The end result shows that 20 Thunnus obesus was infected by 2 different digenean parasites known as Tetrochetus sp. and Rhipidocotyle sp. The prevalence and mean intensity of these digeneans was 62.50% and 6.5 respectively.
Bacteriological study in association with heavy metal tolerance and antibiotic resistance pattern of bacterial population of freshwater giant prawn post larvae
(Terengganu: Universiti Malaysia Terengganu, 2009) Zurina Mohd Dzahari
50 sample bacterial isolates were isolated from Macrobrachium rosenbergii post larvae at marine hatchery of Universiti Malaysia Terengganu. This study were performed to investigate and quantify bacteria colonies, antibiogram, heavy metal and to correlate the relationship of antibiotic and heavy metal resistance with regards to bacterial isolates. There are several agar such as non-selective agar, Trypticase Soy Agar (TSA) and five selective agar which are MacConkey , Thiosulfate Citrate Bile Sucrose (TCBS), Eosin Methylene Blue (EMB),Glutamate Starch Pseudomonas (GSP) and Xylose Lysine Deoxycholate (XLD) were used to isolate the bacteria from post larvae. Results have been showed that bacterial isolates were mostly resistant to Ampicillin and Amoxycillin in antibiotics test by using 15 antibiotics. Based from the total of multiple antibiotic resistant (MAR) Index ; 0.6, had shown that the environment were highly expose to the antibiotics environment such as in water quality. Heavy metal test showed Mercury was successfully can tolerate with all the isolates than Copper. Copper showed the lowest tolerance with the isolates. Bacterial isolates were resistant to antibiotic and heavy metal. The study may provide basis knowledge about the occurrence of heavy metal and antibiotic resistant bacterial strains which may raise preventive measurement on use of antibiotic and awareness on hatchery management.
The effect of dissolved organic matter on survival rate and reproductive performance of Moina macrocopa
(Terengganu: Universiti Malaysia Terengganu, 2009) Zainab Johari
This experiment aims to determine the effect of dissolved organic carbon (OOC) on survival, reproduction, size, growth and number of neonates produce by M macrocopa. Survivorships and average longevity of M macrocopa were affected by DOC. When M macrocopa fed with 0.5% DOC, produces highest number of neonates produce which are about 464 neonates. The 5% of DOC give biggest size of neonates (0.576 mm). The average longevity for three concentrations which are O %, 0.1% and 1% reach to 16 days.
Identification of dna marker in tiger grouper (Epinephelus fuscoguttatus) from Kedah population
(Terengganu: Universiti Malaysia Terengganu, 2009) Wan Mohd Redhuan Wan Azmi
A study to clone and identify DNA marker in Tiger Grouper (Epinephelus fuscoguttatus) in Kedah population was carried out. In this study, DNA from four population of Tiger grouper that from Bali, Kedah, Langkawi and Sabah was extracted from tissue muscles and then used as a template for Polymerase Chain Reaction (PCR) amplification using RAPD-PCR technique. In this PCR Amplification, three types of primer that OPA-01, OPA-11 and OPA-20 had used to produce DNA profile from these four population. Result showed that only one polymorphic fragment sized 504 hp exist from sample E. fuscoguttatus from Kedah population by using primer OPA-01. After that, this fragment was isolated and purified for cloning purpose. In cloning, this fragment was ligated using cloning vector pTZ57R/7 sized 2886 bp and transformation had done using bacteria cell E. coli. Cloning recombinant had successfully got by used plasmid sized -3300 bp. From the result of BLAST showed that there have no significant similarity found and that's mean there have no reported that this sequence have found yet and also this result showed and also maybe it specific for species E. fuscoguttatus. It expected because sequence of E. fuscoguttatus just 0.00002% only compared with model organism zebra fish (Danio rerio ). The future study about this experiment that can design primer according to the cloned sequence and this could be easily and effectively to determine between populations using PCR technique.
Golden apple snail, cassave and maize as alternative feeds for the giant freshwaters prawn, Macrobrachium rosonbergii
(Terengganu: Universiti Malaysia Terengganu, 2009) Syed Yusof Wan Drahman
This study was done to show and to help the public especially to the inferior giant freshwater prawns' farmers about another low cost and easy to find alternative feed, a species known as golden apple snail. Maize and cassava also have been known as alternative feeds among prawn farmers, particularly contain lower protein value than the meat of golden apple snail. Golden apple snail was popular among paddy farmers despite of their ability to destroy and halted the growth of paddy plant. This phenomenal has lead to destruction and low income to the paddy farmers. Four treatments consists of shrimps, each of treatments were fed by raw golden apple snail, maize, cassava and also prawn pellet as control. The result show the specific growth rates of replicates in golden apple snail treatment was 1 % per day, much better than maize and cassava, respectively 0.81 and 0.83% per day. The growth of replicates fed with prawn pellet was the highest, due to its complete set of nutritions. Golden apple snail contain higher amount of protein than maize and cassava that vital for the growth of prawns. Each maize and cassava contains higher amount carbohydrates that important to provide energy for survival. These results suggest that raw golden apple snail meats may represent a commercial environmentally alternative feed, particularly in giant freshwater prawn culture.
Induced thermotolerance of Cyprinus carpio fingerlings following non-lethal heat shock
(Terengganu: Universiti Malaysia Terengganu, 2009) Syarifah Nor Syahirah Syed Muhazani
This study examined the effects of a non-lethal heat shock (NLHS) towards resistance of a subsequent lethal heat stress or in other words, induced thermotolerance (ITT) of the common carp, Cyprinus carpio fingerlings. Prior to testing the ITT, the lethal heat treatment (LHT) of this species was determined. Fish acclimatized at 28°C were heat shocked to 34°C, 36°C, 38°C, 40° C and 42° C for 30 min, and given a subsequent recovery of24 h at 28°C. The survival was determined after 24 h by counting actively swimming animals. Mortality was not observed when fish were heated to 36°C. A slight decrease in survival occurred when fish were exposed to 38°C while 39°C resulted in 50% mortalities, the latter characterized as LT 50. Heat shock at 41 °C killed all animals indicating that this temperature was lethal and thus was considered as the lethal heat treatment (LHT). To test ITT, fingerlings were exposed from 28° C to NLHS of 32° C, 34° C, 36° C and 38°C for 30 min and given a recovery of 8 h. Fingerlings were exposed to LHT for 30 min to determine whether thermotolerance is induced. It is found that all NLHS temperatures confer tolerance of fingerlings against LHT compared to those of the non-heat shocked controls, where total mortalities occurred in the latter treatments. The highest protection was achieved at 38 °C, with an approximately 43% survived incubation, perhaps due to the upregulation of heat shock proteins, a group of protein that are responsible for protection against extreme forms of stress. Further studies such as immunoblotting are required to substantiate the putative role ofHsps on ITT of Cyprinus carpio.
Site-specificity study of monogenean gill parasite in pond culture fish of ikan patin buah (Pangasius nasutus) at Pusat Pengembangan akuakultur Perlok Jerantut, Pahang
(Terengganu: Universiti Malaysia Terengganu, 2009) Surzanne Mohd Agos
From August to September 2008, 30 fishes of Pangasius nasutus (lkan Patin Buah) were collected from the pond culture farm, number BS at Pusat Pengembangan Akuakultur (PPA), Perlok Jerantut Pahang. The fish was used to identify the monogenean gill parasite infecting the gill of Pangasius nasutus (Ikan Patin Buah) in freshwater pond culture in Perlok according to their specific characteristic because monogenea often cause many problems in aquaculture practice all over the world. Monogenean can become a serious threat when fish are crowded together, as in hatcheries and farming operations like pond. This study was performed to determine the site specificity, prevalence,and mean intensity of monogenean in different size classes of Pangasius nasutus in freshwater pond cultures. The condition of the fish and water quality of the pond water were taken. Histological process was carried out to study the structure of the gill and to examine the structural changes on the gill because of the heavily infected by monogenea. Scanning Electron Microscope (SEM) was done to observe more clearly the specimen and the site attachment of the parasite on the gill. This project was conducted in Biodiversity Laboratory, Anatomy Physiology Laboratory and Histology Laboratory at Universiti Malaysia Terengganu
Dissolved oxygen depletion on survival and spawning performance of gourami (Trichogaster trichopterus)
(Terengganu: Universiti Malaysia Terengganu, 2009) Nurul Liyana Mohd Ramly
Dissolved oxygen (DO) depletion commonly occurred in natural environment can cause a series of negative effects in aquatic organsims such as reducing the fish populations, reproduction performances and survival of many fish species. The study of dissolved oxygen depletion on survival and spawning performance of gourami (Trichogaster trichopterus) were conducted for 2 months at the Marine Hatchery Complex of Universiti Malaysia Terengganu. Fifteen pairs of broodstock were exposed to different dissolved oxygen concentration at 1 mg L-1 , 3 mg L- 1 , 5 mg L- 1 , 7 mg L- 1 and 10 mg L- 1 • Hypoxic condition was achieved by introducing the nitrogen gas into the rearing water while high level of dissolved oxygen was achieved by introducing pure oxygen gas into the rearing water. Experimental tank of 1 mg L- 1 , 3 mg L- 1 and 10 mg L- 1 of dissolved oxygen were sealed in order to reduce gas diffusion. There is a significant correlation between dissolved oxygen concentration with survival, spawning performance as well as feed intake in fish. Fish reared in hypoxic condition showed most significant decreased in survival, spawning performance and feed intake while fish exposed in moderate dissolved oxygen concentration especially at 3 mg L- 1 and 5 mg L- 1 shows a significant increased in survival, spawning performance and feed intake.
The morphological study of digenea on mystus numerus in cage culture from Sungai Terengganu
(Terengganu: Universiti Malaysia Terengganu, 2009) Nurul Huda Abdullah
The Mystus nemurus and also known as River Catfish were cultured in cages at Sungai Terengganu. The parasites that infected this species of fish are Platyhelminthes or flatworms, subclass the Digenea of the class Trematode. The digenea is an ndoparasites that have complex life-cycle and can infect fish as intermediate host or definitive host. 30 of River Catfish were examined and the investigated organs were stomach and intestine. This is because the parasite can be found in large number in the intestine and rectum of the fish. The digenean that infected the Mystus nemurus was studied and the morphology was examined to determine the family of the digenean and identify up to genus. The order is Plagiorchiida, superfamily is Opisthorchioidea, family is Heterophyidae, and the genus is Euhaplorchis. The prevalence of River Catfish that infected by these species are 93.33% and the mean intensity are 14.07. The infection of this species influence by many factors such as age of the host, season and population size of the host.
Study of ectoparasite from Clarias gariepinus in cages at Pulau Musang
(2009) Nurul Hafizah Mohamad
This study was conducted at Pulau Musang, Terengganu. A total of 30 fishes were examined in order to obtain the ectoparasites infecting fish from cage culture and to determine the prevalence and mean intensity of ectoparasite found. Only Bychowskyella sp. was found in this research. The prevalence of Bychowskyella sp. found is 86. 7 % and the mean intensity is 6.69 individuals per fish. The Bychowskyella sp. found on various sites of the gills. The gills were divided into four parts which was the first, second, third and fourth gill. Then, the gills were examined under microscope and Bychowskyella sp. was stained with glycerine jelly. The parasite samples were drawn using Camera Lucida to see the detail morphology. The water quality was the main factor to cause fish has parasite and get the disease. So, the farmers must control the water quality in cage culture to decrease the number of fish deaths caused by the disease.
The study of prevalence, mean intensity and morphology of digenea sp. in mystus nemurus fish
(Terengganu: Universiti Malaysia Terengganu, 2009) Nurul Farhana Ramli
The study was done on Mytus nemurus fish in The Tasik Kenyir Reservior. 37 fishes were examined in order to obtain digenean parasite infecting the Mystus nemurus fish and to determine prevalence, mean intensity of digeneans found. The morphological description of the digeneans was also done. The digeneans that were in stomach and intestine have the same morphological structures. So, most probably they are from Euhaplorchis sp. The prevalence and mean intensity of the digenean parasites were high in the intestine of the fish compared to the stomach of the fish. The composition of the digeneans also showed that the digeneans have site specificity at the anterior part of the intestine; site A. Factors like environment, physicology of the digeneans and morphology of the fish probably influence the accumulation of the parasite in Mytus nemurus. Histology part also was done to see the digenean group in the small intestine of the fishes. It was observed that the infection of the digeneans to the Mystus nemurus was not related to the total length of the fish.
Sperm quality of african catfish (Clarias gariepinus) broodstock fed on pellet supplemented with fish oil
(Terengganu: Universiti Malaysia Terengganu, 2009) Nurnajjah Baharom
This study was conducted to determine the effect of pellet supplemented with fish oil on sperm quality of broodstock Clarias gariepinus. Male broodstocks of African Catfish, Clarias gariepinus were divided into two treatments. Treatment A was fed on one experimental diet (fish oil pellet containing 6% fish oil) and Treatment B was fed on one control diet (non-fish oil pellet). The feeding experiment occurred for 6 weeks. The broodstocks were fed twice daily by 1.5% of body weight. Sperm quality was measured by sperm count method using emocytometer during initial (0 days) and final experiment (after 6 weeks). Sperm concentration per ml, total sperm count and milt volume expressed for each treatment was done to compare the sperm quality between both treatments. Result of the study showed that there was an increase in sperm concentration per ml of broodstock in Treatment A from 1.282 ± 0.422 x 1010 to 1.359 ± 0.420 x 1010 whereas in Treatment B, sperm concentration per ml decreased from 8.958 ± 3.424 x 109 to 7.047 ± 2.370 x 109 . Statistical analysis showed no significant difference in sperm concentration for both treatments. The milt volume expressed during initial experiment of both treatments was not significantly different with 4.305 ± 2.493 ml and 4.532 ± 3.261 ml for Treatment A and Treatment B respectively. Treatment B showed higher milt volume after 6 weeks 3.870 ± 3.305 ml compared to Treatment A 2.643 ± 2.609 ml. During the initial experiment, average of total sperm count in each broodstock was 5.377 ± 2.811 x 1010 and 4.186 ± 2.889 x 1010 for Treatment A and Treatment B respectively. After 6 weeks the total sperm count in Treatment A was higher (3.433 ± 2.883 x 1010) compared to Treatment B (2.246 ± 1.534 x 10 10 ). As a conclusion, the sperm quality in broodstock of Clarias gariepinus was not significantly affected by pellet supplemented fish oil possibly because of the concentration of fish oil was not optimum. Increasing the concentration of fish oil might be able to affect the sperm quality significantly. Further study need to be done to look into this aspect. In addition stress might be a factor that can reduce the sperm quality of broodstock during the conditioning process.

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