Pusat Pengajian Sains Marin Dan Sekitaran
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Item Accumulation Of Metallic Elements In Commercial Fish Species Of Setiu Coastal Waters, Terengganu As Pollution Bioindicator(Universiti Malaysia Terengganu, 2018) Muhammad Izzat Bin KamaruzamanThe pollution from Metallic Elements (MEs) becoming one of the major concern in assessing potential human risk on food consumption. Wild commercial fish exposed to the natural environment are the main source of exposure to human. Thus, this study was conducted to determine the concentration of MEs in selected commercial fish of Setiu coastal water and to study the correlation of the metals with the fish sizes. Ten types of MEs including chromium (Cr), manganese (Mn), copper (Cu), zinc (Zn), iron (Fe), nickel (Ni), cadmium (Cd), arsenic (As), lead (Pb) and mercury (Hg) were detected in both the liver and muscle of five commercial species collected namely Auxis sp., Megalaspis cordyla, Rastrelliger sp, Selaroides sp. and Nemipterus sp. Fishes were dissected for muscle and liver and digested using Teflon Bomb digestion method. The concentration of MEs in the organ were detected using the Inductive Coupled Plasma – Mass Spectrophometer. The data obtained show selected MEs were higher in the liver than the muscle except for Cr for all fish species collected. Overall concentration of all fish species shows that the concentration of Fe>Zn>As>Cu>Cd>Mn>Cr>Hg>Ni>Pb in the liver and the concentration of Fe>As>Zn>Cr>Cu>Mn>Ni>Hg>Pb>Cd in the muscle. The size of fish and the trophic level does not influence the MEs concentration in the fish. The concentration of MEs in the liver can safely say that does not affect the concentration of MEs in the muscle means that the fish diet is not the main source of metal content for muscle.Item Analysis Of Bacterial Diversity And Polyhydroxyalkanoate Synthase Genes Associated With Some Marine Sponges(Universiti Malaysia Terengganu, 2018) Amelia Tan Suet MayPolyhydroxyalkanoate (PHA) is scientifically acclaimed as a naturally biosynthesised, biodegradable and biocompatible biopolymer that presents as an alternative to petroleum-based plastic. Recognised as a bacterial hotspot, the marine sponge is an acknowledged resource of novel natural products and genetic material, hence is a desirable target for PHA-producing bacteria or PHA-polymerising genes. However, due to the difficulty in cultivating sponge-associated bacteria plus the emergence of bioinformatics and metagenomics tools, the medically and biotechnologically appealing genes are increasingly exploited by bioengineering prior to biosynthesis. Therefore, in this study, the bacterial diversity and PHA synthase genes (phaC) of locally abundant yet underexplored sponges, explicitly Aaptos aaptos and Xestospongia muta from Pulau Bidong along with A. aaptos from Pulau Redang, were investigated. After metagenome extraction, bacterial diversity was analysed using culture-independent 16S ribosomal ribonucleic acid (16S rRNA) amplicon sequencing. Then, the phaC genes were screened, sequenced, identified and published in the GenBank database. Three phaC labelled 2, 2B and 1B were closely similar to the phaC of Pseudomonas sp., Rhodocista sp., and bacterium AR5-9d_16. Out of ninety-nine dominant genera, twenty-six PHA-producing genera were detected among the three bacterial communities. Geographical factors rather than host species notably influenced sponge-associated bacterial communities.Item The Application Of Two Dimensional Nearshore Spectral Waves (2d-Nsw) Model For Wave Prediction And Wave Power Potential Along Terengganu Coastline(Universiti Malaysia Terengganu, 2014-06) Nur Amalina Binti Abdul LatiffThis thesis is an investigation of the wave power resource in the Terengganu nearshore area. The aim of this study is to evaluate the performance of the nearshore spectra wave model and hence used the simulated result to assess the wave power potential. The MIKE-21 Neashore Spectral Wave (MIKE-21 NSW) model has the potential of serving as a tool for obtaining accurate estimates of wave field condition along the Terengganu nearshore area. Generally, the transformation of the offshore wave field, as it propagates into this area, is computed by using hindcast input wave data from the European Center for Medium Range Weather Forecast‗s (ECMWF) (70 m water depth), as a boundary data. The accuracy of the modelled output was investigated by directly comparing it to the available Acoustic Wave and Current (AWAC) observation wave data (16 m water depth) during the overlapping recording period.Item Biosynthesis Of Polyhydroxyalkanoate By Massilia Haematophila Umtkb-2 Using Optimized Culture Parameters And Its Polymer Characterization(Universiti Malaysia Terengganu, 2018) Jong Tse KiunPolyhydroxyalkanoate (PHA) is a type of biodegradable polymer that is synthesized by some bacteria under limited nutrient and excess carbon source. The production of PHA by Massilia sp. remains mostly unexplored even though some previous studies had highlighted its ability to accumulate P(3HB). In this study, chemical and physical culture parameters were screened for P(3HB) and P(3HB-co-3HV) copolymer production by a local aquatic bacteria, Massilia haematophila UMTKB-2. This strain had shown proficiency in producing homopolymer, P(3HB), using glucose as a carbon source and urea as a nitrogen source. The potential of this bacteria to accumulate copolymer has never been reported. It was found that M. haematophila UMTKB-2 could produce 4.40 g/L of P(3HB-co-3HV) with 4 mol% 3-hydroxyvalerate (3HV) monomer using glucose and 1-pentanol as carbon source and precursor respectively. The P(3HB-co-3HV) production was enhanced through the optimization of fermentation parameters using the response surface methodology (RSM) in shake flasks. The production of P(3HB-co-3HV) by M. haematophila UMTKB-2 using optimal conditions (0.07 wt% of 1-pentanol concentration, 176 rpm of agitation rate, and 122h of incubation time), had yielded 5.0 g/L of P(3HB-co-3HV) with 7 mol% of 3HV monomer, were higher than the value obtained from un-optimized conditions (0.06 wt% of 1-pentanol concentration, 200 rpm of agitation rate, and 108 h of incubation time). In comparison with non-optimized conditions, a 14 % increment of copolymer concentration and two-fold increment of 3HV composition were observed.Item Biosynthesis, Fabrication And Analysis Of Biodegradable Poly(3hydroxybutyrate) And Antimicrobial Agent Blends As Bacterial Control In Aquaculture(Universiti Malaysia Terengganu, 2018) Teh Weng KernBacterial control in aquaculture water system is important because the most prominent problem faced in this system is the high mortality rate caused by uncontrolled bacterial infections. The current measures used to control bacterial infection poses a disadvantage under long term use as it not only affects the environment but humans as well. Poly(3-hydroxybutyrate) [P(3HB)], a bacteria-derived biodegradable polymer was investigated for its potential to act as a matrix for immobilizing antimicrobial agents. The prolonged immobilization of antimicrobial agents on a biodegradable matrix will enable improved inhibition of potentially pathogenic microorganisms in an eco-friendly approach.Item Characterization Of P(3hb-Co-4hb) Copolymer Synthesis Consisting Of High 4hb Monomer Using Cupriavidus Sp. Usmaa1020 Transformant Strains(Universiti Malaysia Terengganu, 2015-08) Muhammad Syafiq Bin IshakPolyhydroxyalkanoates (PHAs) are naturally-occurring biodegradable thermopolyesters accumulated by bacteria. Copolyester of Poly(3- hydroxybutyrate-co-4-hydroxybutyrate) [P(3HB-co-4HB)] were noted for its high biocompatibility. This makes it an excellent candidate for medical as well as biopharmaceutical applications. A Gram-negative bacteria, Cupriavidus sp. USMAA1020 has the ability to synthesized P(3HB-co-4HB) copolymer with various 4HB monomer compositions. Additional copies of PHA synthase gene (PhaC) from Cupriavidus sp. USMAA1020 (PhaC1020) and Cupriavidus sp. USMAA2-4 (PhaC2-4) the key enzyme in PHA synthesis into Cupriavidus sp. USMAA1020 wild-type strain resulted in higher 4HB monomer composition of the copolymer produced. The transformant strains was cultivated with 1,6-hexanediol (0.5175 wt%) and γ-butyrolactone (0.1725 wt%) through one-stage cultivation. 4HB composition up to 93 mol% obtained by Cupriavidus sp. USMAA1020phaC1020 while 91 mol% 4HB molar fraction was obtained by Cupriavidus sp. USMAA1020phaC2-4.Item Characterization of p(3hb-co-4hb) copolymer synthesis consisting of high 4hb monomer using cupriavidus sp. usmaa1020 transformant strains(Terengganu: Universiti Malaysia Terengganu, 2015) Muhammad Syafiq bin IshakPolyhydroxyalkanoates (PHAs) are naturally-occurring biodegradable thermopolyesters accumulated by bacteria. Copolyester of Poly(3-hydroxybutyrate-co-4-hydroxybutyrate) [P(3HB-co-4HB)] were noted for its high biocompatibility. This makes it an excellent candidate for medical as well as biopharmaceutical applications. A Gram-negative bacteria, Cupriavidus sp. USMAA1020 has the ability to synthesized P(3HB-co-4HB) copolymer with various 4HB monomer compositions.Item Community Structures And Behavioural Ecology Of Indo – Pacific Humpback Dolphins (Sousa Chinensis) In Cowie Bay, Sabah, Malaysia(Universiti Malaysia Terengganu, 2014) Hairul Masrini Binti MuhamadLittle is known about the Indo-Pacific humpback dolphins that inhabit Cowie Bay, Sabah. Therefore, this project is expected to provide scientific information on Indo-Pacific humpback dolphin„s group size, social structure and residence patterns in Cowie Bay, Sabah. Boat based surveys were conducted from April 2008 to March 2009 to evaluate the status of humpback dolphins using two methods; Peterson capture-recapture method (using photo identification) and direct count method. Data were analysed using statistical software and Microsoft Photo Manager. Results showed that the humpback dolphin‟s community was small. Only three individuals were identified and catalogued living in the bay within 1.8 – 25 meters depth of water. The sighting rate was higher during neap tides than during spring tides. However, the group size (Mean = 2.49) was not affected by the different types of tides (U = 250.5, p > 0.05, r = -0.12). The community was recorded to be socially active in the afternoon compared to morning and evening.Item Community Structures And Behavioural Ecology Of Indo – Pacific Humpback Dolphins (Sousa Chinensis) In Cowie Bay, Sabah, Malaysia(Universiti Malaysia Terengganu, 2014) Hairul Masrini Binti MuhamadLittle is known about the Indo-Pacific humpback dolphins that inhabit Cowie Bay, Sabah. Therefore, this project is expected to provide scientific information on Indo-Pacific humpback dolphin‘s group size, social structure and residence patterns in Cowie Bay, Sabah. Boat based surveys were conducted from April 2008 to March 2009 to evaluate the status of humpback dolphins using two methods; Peterson capture-recapture method (using photo identification) and direct count method. Data were analysed using statistical software and Microsoft Photo Manager. Results showed that the humpback dolphin’s community was small. Only three individuals were identified and catalogued living in the bay within 1.8 – 25 meters depth of water. The sighting rate was higher during neap tides than during spring tides. However, the group size (Mean = 2.49) was not affected by the different types of tides (U = 250.5, p > 0.05, r = -0.12). The community was recorded to be socially active in the afternoon compared to morning and evening. The analysis showed that the percentage for the individuals to be seen together was 48.8%, and the range of 9 – 12% observed to be solitary sightings. The association indices between individuals suggested that the community is highly associated with each other (AI = 0.6373 / 0.5756 / 0.6728).Item Distribution and fluxes of chlorophyll-a and dissolved nitrogen and phosphorus from Terengganu river estuary to South China Sea(Terengganu: Universiti Malaysia Terengganu, 2014-02) Suryani SulongThis study is to determine the distribution, flux and relationship of dissolved nitrogen, dissolved phosphorus and chlorophyll-a (productivity), that are then used to develop the dissolved nitrogen, dissolved phosphorus and productivity (chlorophyll-a) fluxes model in the Terengganu River Estuary using the Land-ocean Interactions in the Coastal Zones (LOICZ) Biogeochemical Modelling Guidelines. Dissolved nitrogen and phosphorus and chlorophyll-a water samples were collected from 16 sampling stations in the rivers (Terengganu River and Nerus River), estuary and coastal water during the Northeast, Southwest and Inter-monsoon seasons as well as during low and high tides from January to November 2010.Item Distribution And Local Knowledge Of Freshwater Chelonian In Terengganu With Special Emphasis On Population Size Determination Of Dogania Subplana(Universiti Malaysia Terengganu, 2018-09) Noor Shahirah Mohd IbrahimThe distribution and local status of most freshwater turtles and tortoises (FFTs) in Peninsular Malaysia is not well documented. Human conflict has become a major threat to wildlife, thus study involving local communities was carried out to assess the level of knowledge and awareness on FTTs. Since the populations of FTTs are facing a serious global decline due to human activities, population studies are important to assess their status and dynamics. Hence, this study intended to provide assembled information regarding the (i) distribution of FTTs in Terengganu, (ii) local knowledge and awareness level, and (iii) population size estimation of Dogania subplana for future conservation efforts. Distribution data of FTTs were collected from published materials and unpublished data since 1999 to 2016 together with the current surveys. Then, demographics of the locals were surveyed altogether with their knowledge and awareness on FTTs. Mark recapture technique was applied for two consecutive years to determine the population size and distribution with additional findings on growth rate and movement range of D. subplana population in Sekayu lowland forest. Terengganu supports 68 percent (13 species) of FTTs from Peninsular Malaysia. There were more species expected to be found in Terengganu since habitats availability are suitable for some species that were not found in this study.Item Distribution Of Water Physico-Chemical Parameters And Biogenic Elements In Setiu Lagoon During Monsoon Events As Indicator For Primary Productivity(Universiti Malaysia Terengganu, 2015-06) Aida Royyani Binti Abd Ariff; School of Marine and Environmental Scienceshe study was conducted in the Setiu Lagoon, Terengganu involving three sampling periods, namely in May 2007 (post-monsoon), September 2007 (pre-monsoon) and January 2008 (monsoon). Samples of nutrients (NO3 - - - + N, NO2 -3 -N, NH4 -N, PO4 -P), primary productivity, chlorophyll-a and physico-chemical parameters of water were collected during low and high tides from 6 stations in the lagoon area and 6 stations in the riverine area except for primary productivity where samples were taken during the pre- monsoon and monsoon seasons only during high tide. Data obtained were analyzed to assess the variations of water quality within Setiu lagoon spatially and temporally subjected to monsoonal events and tidal changes. From the results, the N:P ratio indicated that the study area was mostly N limited especially in the lagoon area. Correlation analysis indicated that phytoplankton consumed more NO3 - -N during low tide in the post- monsoon (p<0.01) and pre-monsoon season (p<0.05) but during high tide, PO4 -P more favorable to phytoplankton in the post-monsoon (p<0.01) and pre-monsoon season (p<0.01). Factor analysis showed that monsoonal event affects the phytoplankton productivity in the study area compared to tidal event. Temperature was the primary factor while nutrients concentration and salinity were the secondary factors. These indicated that monsoonal events coupled with tidal events contribute to the nutrient supply into the study area. Non-point sources and natural sources were the contributer for the nutrient enhancement in the study area. The study area was still not polluted with nutrients loading and did not exhibit any sign of eutrophication. The nutrient level did not exceed the standard of MWQCS (2010). The physico-chemical parameters and nutrients can be a good indicator for primary productivity in the Setiu lagoon.Item Distribution of water physico-chemical parameters and biogenic elements in Setiu lagoon during monsoon events as indicator for primary productivity(Terengganu: Universiti Malaysia Terengganu, 2015-06) Aida Royyani binti Abd AriffThe study was conducted in the Setiu Lagoon, Terengganu involving three sampling periods, namely in May 2007 (post-monsoon), September 2007 (pre-monsoon) and January 2008 (monsoon).Item Distribution of water physico-chemical parameters and biogenic elements in Setiu lagoon during monsoon events as indicator for primary productivity(Terengganu: Universiti Malaysia Terengganu, 2015) Aida Royyani binti Abd AriffThe study was conducted in the Setiu Lagoon, Terengganu involving three sampling periods, namely in May 2007 (post-monsoon), September 2007 (pre-monsoon) and January 2008 (monsoon). Samples of nutrients (NO3--N, NO2--N, NH4+-N, PO4-3-P), primary productivity, chlorophyll-a and physico-chemical parameters of water were collected during low and high tides from 6 stations in the lagoon area and 6 stations in the riverine area except for primary productivity where samples were taken during the pre-monsoon and monsoon seasons only during high tide. Data obtained were analyzed to assess the variations of water quality within Setiu lagoon spatially and temporally subjected to monsoonal events and tidal changes. From the results, the N:P ratio indicated that the study area was mostly N limited especially in the lagoon area. Correlation analysis indicated that phytoplankton consumed more NO3--N during low tide in the post-monsoon (p<0.01) and pre-monsoon season (p<0.05) but during high tide.Item The Distributions And Behaviour Of Nitrogen-Based Nutrients In Terengganu River Estuary(Universiti Malaysia Terengganu, 2016-04) Azyyati Binti Abdul AzizThis study was carried out to determine the distribution and behaviour of nitrogen (N)-based nutrients for surface water in Terengganu River estuary (TRE). The N- based nutrients measured were dissolved inorganic N (nitrite, nitrate and ammonia) and organic N (dissolved organic nitrogen (DON) and particulate organic nitrogen (PON)). Surface water samples were collected during longitudinal and diurnal surveys under different tidal conditions from February to September 2012. Low concentration of nitrite (0.002-0.393 µM) and nitrate (0.3-4.7 µM) were found during longitudinal surveys. Meanwhile, the concentration of ammonia, DON and PON were 1.0-35.4 µM, 3.2-40.6 µM and 1.5-23.6 µM, respectively. The results also indicated that the percentage concentrations of dissolved inorganic N (longitudinal = 35%; diurnal = 19%) were lower than organic N (longitudinal = 65%; diurnal = 81%). Generally, distributions of N-based nutrients were higher in freshwater region which indicated a strong riverine influenced in TRE. In addition, most of N-based nutrients showed higher concentration during spring tide suggesting contribution from the resuspension of bottom sediments. Most of the parameters measured appear to behave non-conservatively with an addition tendency.Item The effect of plant hormones on oil accumulation, fatty acid composiitons and expression levels of fatty acid biosynthetic genes in the marine mircoalga, chlorella vulgaris(Terengganu: Univsrsiti Malaysia Terengganu, 2014-05) Malina JusohMicroalgae lipids and oils are potential candidates for renewable-biofuels and nutritional purposes. It has long been known that many microalgae species accumulate substantial amount of lipids and oils under environmental stresses, presumably by channelling the photosynthesis product originally allocated to support cell division and growth to the production of energy-rich storing molecules.Item Effects of elevated tropospheric ozone on biochemical activities and yield of selected indica rice plants(Terengganu: Universiti Malaysia Terengganu, 2015-07) Azrin binti SurotoOzone had been recognized as one of the major threats towards crops, including rice by giving the adverse effects on biochemical activities, physiological performance and yield production. In Malaysia, anthropogenic sources are rapidly increasing and this condition had caused increased ozone formation.Item Effects of elevated tropospheric ozone on biochemical activities and yield of selected indica rice plants(Terengganu: Universiti Malaysia Terengganu, 2015) Azrin binti SurotoOzone had been recognized as one of the major threats towards crops, including rice by giving the adverse effects on biochemical activities, physiological performance and yield production. In Malaysia, anthropogenic sources are rapidly increasing and this condition had caused increased ozone formation. Domestic rice consumption is projected to be high due to population increase. High production of rice is targeted from the existing rice planting areas. Since there is no plan by the government to increase the area for rice cultivation, there is an urgent need to identify the most tolerant cultivar to ozone.Item ELUCIDATION OF POTENTIAL PROTEINS ASSOCIATED WITH Vibrio parahaemolyticus INFECTION IN WHITELEG SHRIMP, Penaeus vannamei USING HOST-PATHOGEN PROTEIN-PROTEIN INTERACTION NETWORK ANALYSIS(Universiti Malaysia Terengganu, 2024) NUR FATHIAH ROSILANItem Fabrication and characterization of gellan gum films incorporating nanostructured titanium dioxide for wound dressing applications(Universiti Malaysia Terengganu, 2019) Nur Arifah Binti IsmailTitanium dioxide (TiO2) nanostructures, which are nanoparticles, nanorods and nanotubes, were successfully synthesised by using the hydrothermal method. These TiO2 nanostructures were incorporated into gellan gum (GG) film. Good film was produced by using 1.00 g GG with a 5 mM CaCl2 cross-linker and 50% w/w glycerol as a plasticiser dried at 50 °C for 24 h. GG incorporating TiO2 nanoparticles (GG+TiO2-NPs), nanorods (GG+TiO2-NRs) and nanotubes (GG+TiO2-NTs) was produced via the evaporative casting method. GG+TiO2-NTs film shows the best mechanical properties, a high swelling ability and moderate water vapour transmission rates attributed to the chemical interaction between GG and TiO2 nanotubes. GG+TiO2-NTs film also shows antibacterial properties with 10 ± 0.38 mm, 12 ± 0.06 mm, 11 ± 0.06 mm and 10 ± 0.12 mm inhibition zone was obtained against Staphylococcus aureus, Streptococcus, Escherichia coli and Pseudomonas aeruginosa, which are as good as control samples (penicillin).
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