Pusat Pengajian Sains Marin Dan Sekitaran

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Browsing Pusat Pengajian Sains Marin Dan Sekitaran by Subject "Amelia Tan Suet May"

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    Analysis Of Bacterial Diversity And Polyhydroxyalkanoate Synthase Genes Associated With Some Marine Sponges
    (Universiti Malaysia Terengganu, 2018) Amelia Tan Suet May
    Polyhydroxyalkanoate (PHA) is scientifically acclaimed as a naturally biosynthesised, biodegradable and biocompatible biopolymer that presents as an alternative to petroleum-based plastic. Recognised as a bacterial hotspot, the marine sponge is an acknowledged resource of novel natural products and genetic material, hence is a desirable target for PHA-producing bacteria or PHA-polymerising genes. However, due to the difficulty in cultivating sponge-associated bacteria plus the emergence of bioinformatics and metagenomics tools, the medically and biotechnologically appealing genes are increasingly exploited by bioengineering prior to biosynthesis. Therefore, in this study, the bacterial diversity and PHA synthase genes (phaC) of locally abundant yet underexplored sponges, explicitly Aaptos aaptos and Xestospongia muta from Pulau Bidong along with A. aaptos from Pulau Redang, were investigated. After metagenome extraction, bacterial diversity was analysed using culture-independent 16S ribosomal ribonucleic acid (16S rRNA) amplicon sequencing. Then, the phaC genes were screened, sequenced, identified and published in the GenBank database. Three phaC labelled 2, 2B and 1B were closely similar to the phaC of Pseudomonas sp., Rhodocista sp., and bacterium AR5-9d_16. Out of ninety-nine dominant genera, twenty-six PHA-producing genera were detected among the three bacterial communities. Geographical factors rather than host species notably influenced sponge-associated bacterial communities.