(Terengganu: Kolej Universiti Sains dan Teknologi Malaysia, 2005) Abdul Rahman Hasim
The effect of lipase specificity on transesterification of palm olein with cod liver oil was studied using 5 different lipases from Rhizomucor miehei, Aspergillus niger, Pseudomonas fluorescens, wheat germ and Amano lipase PS-C 1. The transesterification reaction was carried out at 60°C and 200 rpm for 6 hours in watersaturated hexane. Each lipases catalytic performance was appraised by determining the changes in peak composition and concentrations using Reversed-Phase High Performance Liquid Chromatography (RP-HPLC) as well as calculated degree of
hydrolysis (DoH) and degree of transesterification (DoT).