Effect of lipase specificity on the transesterification of palm olein with cod liver oil
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Date
2005
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Terengganu: Kolej Universiti Sains dan Teknologi Malaysia
Abstract
The effect of lipase specificity on transesterification of palm olein with cod liver oil was studied using 5 different lipases from Rhizomucor miehei, Aspergillus niger, Pseudomonas fluorescens, wheat germ and Amano lipase PS-C 1. The transesterification reaction was carried out at 60°C and 200 rpm for 6 hours in watersaturated hexane. Each lipases catalytic performance was appraised by determining the changes in peak composition and concentrations using Reversed-Phase High Performance Liquid Chromatography (RP-HPLC) as well as calculated degree of
hydrolysis (DoH) and degree of transesterification (DoT).
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LP 1 FST 1 2005