Development of agrobacterium-mediated transformation method for marine microalgae (Chlorella sp. and Nannochloropsis sp.)

dc.contributor.authorWilly, Yee
dc.date.accessioned2012-10-08T03:43:16Z
dc.date.available2012-10-08T03:43:16Z
dc.date.issued2011-02
dc.description.abstractAn important step in the development of an efficient Agrobacterium-medialed transformation system is the establishment of optimal conditions for efficient TDNA transfer. Parameters known to influence the efficacy of Agrobacterium tumefaciens T-DNA transfer such as pre-culture of algal cells, inoculum density, cocultivation temperature, co-cultivation duration, pH of co-cultivation medium and the concentration of acetosyringone were evaluated by monitoring transient GUS expression two days post-infection.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/1967
dc.language.isoenen_US
dc.publisherTerengganu : Universiti Malaysia Terengganuen_US
dc.subjectQR 82 .R45 W5 2011en_US
dc.subjectWilly, Yeeen_US
dc.subjectTesis FST 2011en_US
dc.subjectAgrobacteriumen_US
dc.titleDevelopment of agrobacterium-mediated transformation method for marine microalgae (Chlorella sp. and Nannochloropsis sp.)en_US
dc.typeThesisen_US
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