Development of agrobacterium-mediated transformation method for marine microalgae (Chlorella sp. and Nannochloropsis sp.)
Loading...
Date
2011-02
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Terengganu : Universiti Malaysia Terengganu
Abstract
An important step in the development of an efficient Agrobacterium-medialed
transformation system is the establishment of optimal conditions for efficient TDNA
transfer. Parameters known to influence the efficacy of Agrobacterium
tumefaciens T-DNA transfer such as pre-culture of algal cells, inoculum density, cocultivation
temperature, co-cultivation duration, pH of co-cultivation medium and the concentration of acetosyringone were evaluated by monitoring transient GUS
expression two days post-infection.
Description
Keywords
QR 82 .R45 W5 2011, Willy, Yee, Tesis FST 2011, Agrobacterium