Isolation of polyhydroxyalkanoate producing bacteria from sediment collected at Setiu
Loading...
Date
2013
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Universiti Malaysia Terengganu
Abstract
Gram negative strains, A 22 l o-
6 l and A 22 l o-
6 2 were isolated from
mangrove sediment collected at Setiu. Both strains are potential
polyhydroxyalkanoate (PHA) producer after shown positive results in screening for
PHA production with Nile red stain when emitted pink fluorescence under UV light.
Biosynthesis of PHA by both strains were carried out with glucose, fructose and oleic
acid as carbon sources. GC analysis on dried sample of both strains from biosynthesis
enriched with glucose and fructose detected production of medium chain length PHA.
Strain A22 10-6 1 detected in production of C io, poly(3-hydroxydecanoate) P(3HD),
C 12, poly(3-hydroxydodecanoate) P(3HD0) and C 14 , poly(3-hydroxytetradecanoate)
P(3HTD) while A 22 1 o-6 2 detected for production of P(3HD) and P(3HTD).
Description
Keywords
LP 18 FMSM 2 2013, Muhammad Amirrul Rasyid Ahmad