Identification of bacteria isolated on TCBS agar from the white leg shrimp litopenaeus vannamei cultured in the west coast of penisular Malaysia
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Date
2014
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Universiti Malaysia Terengganu
Abstract
In this present study, eight isolates of bacteria associated with manne shrimp
aquaculture were collected from the species Litopenaeus vannamei. Mysis and water
samples were collected at Gertak Sanggul Aquaculture farm and inoculated on
Thiosulphate-Citrate-Bile Salts agar (TCBS) for 72 hours at 37 °C . All of the strains
were identified by biochemical test which is Gram staining, followed by 16S
ribosomal RNA gene sequence analysis. All the isolated were cultured on
Thiosulphate-Citrate Bile Sucrose agar which have been accepted as the selective
medium for isolation of Vibrio species. .
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Shahzia Shah Rasool, LP 43 PPSMS 1 2014