Detection of aquatic pollutants using luminescent bactteria
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Date
2014
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Universiti Malaysia Terengganu
Abstract
Nowadays, luminescent bacteria as a sensing organism to detect the aquatic pollutants
have been widely used. In this study, luminescent bacteria were identified and the effect
of different concentrations of pollutants towards the density of luminescent bacteria was
carried out. The luminescent bacteria were isolated from the ink of the squids. Then, the
Gram staining was done to observe the shape of the luminescent bacteria. The bacteria
were identified using VITEK. Besides that, the bacteria were tested with different NaCl
concentration (0, 1, 2, 3, 4 and 5 % (w/v)) and at temperature (4, 26, 28, 37 and 40
°
C).
Then, toxicity test was carried out with 1.5 ml of toxicants Cd, Zn Cb, HgS04,
Pb(N03)2,
diazinon and malathion (2, 4, 6, 8 and 10 ppm) were added to 1.5 ml of bacterial
suspension in a cuvette. As for control, 1.5 ml of saline solution was added to 1.5 ml of
bacterial suspensions.
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Aty Atiqah Muhassan, LP 3 PPSMS 1 2014