Identification of tenualosa SP. in Perak Riever using morphometric measurement, meristic count and coi gene analysis
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Date
2014
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Universiti Malaysia Terengganu
Abstract
Tenualosa tali and Tenualosa macrura can be found in Sarawak water and have
higher commercial value in Malaysia (Blaber et al., 1996; Phillip, 2001). According
to Perikanan (2012), Tenualosa sp. also can be found in Perak River, but the species
of this genus still 1midentified. All samples were collected using net in December
2013 and February 2014 and the species was identified based on distinguished
character of morphological and meristic count. DNA was extracted from five sample
of Tenualosa sp. PCR amplification of 600 bp partial fragment of CO 1 gene was
amplified using CO 1 marker. This PCR fragment was then sequenced and analysed
using Mega version 5. The result from BLAST showed that there are the sequences
have high similarity with CO 1 gene. The phylogenetic analysis was constructed using
Neighbour Joining tree and Maximum Likelihood tree.
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Muhammad Faiz Zakaria, LP 16 PPSMS 1 2014