Agrobacterium-mediated transformation of omega-3 desaturase gene into rice calli

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dc.contributor.authorSiti Mohainim Mohammad
dc.date.accessioned2018-11-18T03:42:02Z
dc.date.available2018-11-18T03:42:02Z
dc.date.issued2013
dc.description.abstractC urrent s tu dy is an atempt to trans fo rm the Omega-3 desaturase gene into rice calli using Agrobacterium-mediated tec hniq ue. The effect of vani llin concentrations at 0, 200 and 400 mM o n the rate of trans fo rmation was examined. Agrobacterium lumerfaciens LBA 4404 ha rbo uring Omega-3 desaturase gene and hygromycin phosphotransferase gene (hp t) conferring resistance to hygromycin was used as marker and selection o f puta tive transforman t. GUS histochemical assay provided the first evi de nce o f the transgene and futher confirmed using PCR analysis. 400 rnM o f va ni llin showed the sig ni ficant e ffect i n rice growth and trans fo rmation e fficiency.en_US
dc.identifier.urihttps://umt-ir.umt.edu.my/xmlui/handle/123456789/9969
dc.language.isoenen_US
dc.publisherUniversiti Malaysia Terengganuen_US
dc.subjectLP 5 FST 1 2013en_US
dc.subjectSiti Mohainim Mohammaden_US
dc.titleAgrobacterium-mediated transformation of omega-3 desaturase gene into rice callien_US
dc.typeWorking Paperen_US
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