Efficacy of nucleotide related products on growth, blood chemistry, oxidative stress and growth factor gene expression of juvenile red sea bream, Pagrus major
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Date
2016
Journal Title
Journal ISSN
Volume Title
Publisher
Aquaculture
Abstract
A feeding trial was conducted to determine the efficacy of nucleotide related products on growth, blood chemistry,
oxidative stress and growth factor gene expression of juvenile red sea bream. Five experimental diets were
formulated to contain 49% protein, 11% lipid and the diet without nucleotide related product supplementation
was the control (D1). Nucleotide related products like; nucleoside by-products (NBPs) and inosine were supplemented
at 1, 3 and 0.03, 0.1% consecutively with basal ingredients of D1 and named as D2, D3 & D4 and D5 respectively.
Experimental diets were fed to triplicate groups of fish for 60 days. Fish fed diet D5 showed
significantly the highest final bodyweight and %weight gain followed by the diet groups D2 and D4. Fish fed control
group showed the lowest growth performance and were not differed significantly with diet group D3. Feed
conversion ratio and protein efficiency ratio were also significantly higher in diet group D5, whereas the other
supplemented group showed intermediate value. A wide variation in some of the blood parameters was observed.
In case of oxidative stress parameters, fish fed inosine supplemented diets showed the best conditions because
they performed better under oxidative stress conditions as well as had the highest tolerance against
oxidation.AmongNBP supplemented groups, diet group D2 also showed acceptable conditions of oxidative stress
tolerance. Stress resistance against lowsalinity exposure (LT50) also increasedwith dietary supplementation and
it was significantly the highest in fish fed diet group D5. Total serum protein, serum lysozyme activity and total
peroxidase content tended to be higher (P N 0.05) in NBP and inosine supplemented diet groups. In numerically
higher hepatic IGF-1, mRNA expression was found in diet groups D2 and D4. However, IGF-1 and IGF-2 mRNA
expressions were not significantly altered by dietary supplementations in the present study. Considering overall
performance of the present study, we concluded that inosine and low concentration of NBP (1%) could be effectively
used as dietary supplements for better growth and health performance of Pagrus major.
Statement of relevance: In this study industrial by-products, nucleoside by-products and relatively lowcost nucleoside,
inosine has been evaluated as potential functional nutrients for marine fish such as red sea bream. Utilization
of these functional supplements will help to reduce nucleotide administration cost in fish feed as well as to
develop low fishmeal based functional aquafeed in the near future.
Description
Keywords
Nucleoside by-product, Inosine, Oxidative stress, Growth, Blood chemistry, Red sea bream
Citation
Vol.646; 8-16 p.