Studies on the polysaccharide-producing bacteria, shewanella putref aciens isolated from marine sponge, theonella sp.
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Date
2015
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Terengganu: Universiti Malaysia Terengganu
Abstract
Marine sponge, Theonella sp. is found in great quantity from Malaysian’s
ocean. Theonella sp. are one of marine sponges that produces a significant
bioactive compounds. They are stated to be potential drug lead producers
having exceptional structures that are unavailable from terrestrial region.
Marine bacteria associated with sponges are significance as new promising
source of large amount of biologically active compounds. The aims of this
study were to identify polysaccharide-producing bacteria isolated from
Theonella sp., to screen selected bacterial polysaccharides for antibacterial
and toxicity and to determine composition of selected bacterial
polysaccharide, Shewanella putrefaciens. Subsequent to this, bacterial
sampling of Theonella sp. from Bidong Island in Terengganu was conducted.
A total of seven types of bacteria were identified as Shewanella putrefaciens,
Brevundimonas diminuta, Alcaligenes faecalis, Burkholderia cepacia,
Fusobacterium varium, Capnocytophaga sp. and Vibrio damsela. S.
putrefaciens, Capnocytophaga sp., B. diminuta and B. cepacia were
determined as polysaccharide-producing bacteria. Disc diffusion test and
brine shrimp lethality test have shown that only S. putrefaciens crude
polysaccharide gave antibacterial activity and also toxic towards brine
shrimp, Artemia salina. Composition analysis determined S. putrefaciens
crude and acidic polysaccharides were hetero polysaccharide consisted of
glucose, trehalose and mannose, respectively. Only crude polysaccharide
contained trace elements of sulphur and uronic acid with molecular weight of
250.5 kDA. The presence of suphur and uronic acid in S. putrefaciens crude
polysaccharide have been significantly ascertained to be sulfated
polysaccharide. The presence of trace elements associated with
polysaccharide gave positive result and potential value towards medicinal
and pharmaceutical purposes.
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Keywords
QP 702 .P6 L8 2015, Lukman Hakim bin Mohd Din, Polysaccharides