A simple and efficient total genomic DNA extraction method for individual zooplankton

Simple item page
dc.contributor.authorHanafiah Fazhan
dc.contributor.authorKhor Waiho
dc.contributor.authorMd. Sheriff Shahreza
dc.date.accessioned2017-04-09T08:02:50Z
dc.date.available2017-04-09T08:02:50Z
dc.date.issued2015-05
dc.description.abstractMolecular approaches are widely applied in species identification and taxonomic studies of minute zooplankton. One of the most focused zooplankton nowadays is from Subclass Copepoda. Accurate species identification of all life stages of the generally small sized copepods through molecular analysis is important, especially in taxonomic and systematic assessment of harpacticoid copepod populations and to understand their dynamics within the marine community. However, total genomic DNA (TGDNA) extraction from individual harpacticoid copepods can be problematic due to their small size and epibenthic behavior. In this research, six TGDNA extraction methods done on individual harpacticoid copepods were compared. The first new simple, feasible, efficient and consistent TGDNA extraction method was designed and compared with the commercial kit and modified available TGDNA extraction methods. The newly described TGDNA extraction method, “Incubation in PCR buffer” method, yielded good and consistent results based on the high success rate of PCR amplification (82%) compared to other methods. Coupled with its relatively consistent and economical method the “Incubation in PCR buffer” method is highly recommended in the TGDNA extraction of other minute zooplankton species.en_US
dc.identifier.citationVol.5(1);en_US
dc.identifier.issn21931801
dc.identifier.urihttp://hdl.handle.net/123456789/5442
dc.language.isoenen_US
dc.publisherSpringerPlusen_US
dc.subjectTGDNA extractionen_US
dc.subjectCopepoden_US
dc.subjectPCRen_US
dc.subjectHarpacticoiden_US
dc.subjectPCR bufferen_US
dc.subjectVivantisen_US
dc.titleA simple and efficient total genomic DNA extraction method for individual zooplanktonen_US
dc.typeArticleen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
J2016-12-A simple and efficient total genomic DNA extraction method for individual zooplankton.pdf
Size:
1.06 MB
Format:
Adobe Portable Document Format
Description:
Full taxt
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections