INHIBITIVE CHEMICAL CUE OF Pseudomonas pseudoalcaligene ON BIODEGRADATION OFANTHRACENE IN SEAWATER MEDIUM
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Date
2009
Journal Title
Journal ISSN
Volume Title
Publisher
Journal of Sustainability Science and Management
Abstract
This study aims to reveal the self-inhibitive mechanism of an oil degrading bacteria,
Pseudomonas pseudoalcaligene biodegradation of anthracene in seawater. Biodegradation
of anthracene by the oil-degrading bacteria was analogue to the Monod equation and exhibited
a typical bacterial growth pattern. Biodegradation of anthracene was retarded when the bacteria
approached its stationary growth phase. The results indicated that the level of dissolved organic
carbon and hydrogen ion in the seawater mediwn increased due to anthracene biodegradation.
The organic compounds were believed to be secondary metabolites produced by the oil-degrading
bacteria. These metabolites seem to inhibit activities of the oil degrading bacteria. The inhibitive
effect was confirmed when the newly inoculated oil degrading bacteria failed to grow in the
residue mediwn replenished with nutrients and new carbon source and incubated under optimal
condition. It is interesting to note that, this replenished mediwn supported the growth of other
non-oil degrading bacteria, Erythrobacter citreus. Presence of E. citreus in the mediwn utilized
the inhibitive metabolites and thus reswned activities of the oil degrading bacteria for anthracene.
Mixing the oil-degrading bacteria (P. pseudoalcaligenes) and non-oil degrading bacteria (E. citreus)
improved biodegradation ofanthracene in the seawater. The bacterial mixture improved anthracene
degradation by 36% compared to the oil degrading bacteria alone.
Description
Keywords
Anthracene, n-octadecane, Biodegradation, Mixed bacterial culture, Inhibition