Browsing by Author "SAW HONG LOH"

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    EFFECT OF NITRATE ON OIL CONTENT AND FATTY ACID COMPOSITON OF Nannochloropsis SP. AT EARLY STATIONARY GROWTH PHASE
    (Journal of Sustainability Science and Management, 2012) THYE SAN CHA; FONG LEE NG; AHMAD AZIZ; SAW HONG LOH
    A study was conducted to investigate the effect of different nitrate concentrations (0.03,0.05, 0.09, 0.18, 0.34 and 0.66 mM) on oil content and fatty acid composition of Nannochloropsis sp. (strain UMT-M3) at early stationary growth phase. Results showed that the biomass production increased approximately 7-folds at 0.34 and 0.66 mM nitrates (p>0.05), which produced between 0.63 to 0.78 g V of cells dry weight. The highest oil content of 13.3% (dry weight basis) was obtained at 0.34 mM nitrate. The analysis of oil revealed that palmitic (C16:0), stearic, (C18:0),oleic (C18:1), linoleic (C18:2), gamma-linolenic (C18:3n6) and alpha-linolenic (C18:3n3) acids were the major type of fatty acids detected in all nitrate treatments. Interestingly, the accumulation pattern for C16:0 was completely in reverse of C18:2 and C18:3n3 in nitrate, ranging from 0.03 to 0.18 mM. The most critical regulatory point occurred at 0.05 mM nitrate, where the highest (p