Browsing by Author "Nurul Hazirah Mat Lazim"

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    Phenyl ethanone and sterols from Acanthaster planci as potential PPAR-ligand
    (Journal of Chemical and Pharmaceutical Research, 2015) Nurul Hazirah Mat Lazim; Asnuzilawati Asari; Faridah Mohamad; Tg. Muhammad T. S.; Noraznawati Ismail; Mariam Taib; Aziz Ahmad; Habsah Mohamad
    A new phenyl ethanone (1-[4-(1,4-diamino-heptyl)-phenyl]-ethanone (1))and two known sterols (stigmasterol (2) and β–sitosterol (3))were succesfully isolated from methanolic extract of Acanthaster planci collected from the East Coast of Peninsular Malaysia. The chemical structures of compounds 1-3 were deduced on the basis of extensive spectral data (1D and 2D NMR), MS and IR spectroscopy techniques; as well as in comparison the data with those reported in the literature.Compounds 1-3were not toxic towards HepG2 cells and displayed as a potential peroxissome proliferator activated receptor (PPAR) ligand.
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    Potential Anti-Atherosclerotic Compound Isolated from Acanthaster planci
    (Research Journal of Pharmaceutical, Biological and Chemical Sciences, 2016) Nurul Hazirah Mat Lazim; Asnuzilawati Asari; Faridah Mohamad; Tg. Muhammad TS; Noraznawati Ismail; Aziz Ahmad; Mariam Taib; Habsah Mohamad
    Methyl benzoate was isolated from the the outer layer of A. planci (APOL) and tested towards cytotoxicity to HepG2 cells and Luciferase assay. The compound was not toxic towards HepG2 cells with IC50 value of 150.71 ±11.31 μg/ml. Methyl benzoate was screened with PPRE promoter to determine its ability as PPAR ligand and expressed the target gene with maximum fold change of range 3.5 to 4.0 at concentration 0.78 μg/ml. The confirmation as PPARɤ ligand was carried out with SRB-1 promoter and the positive results were obtained. The compound showed optimum expression higher than positive control used at 3.12μg/ml with almost 5.0 fold change. In addition, expression of Luciferase genes was higher than positive control even at lower concentration 0.39 μg/ml. These results indicated that methyl benzoate from APOL have the potential as anti-atherosclerotic agent, might as well beneficial as pharmaceutical products to prevent atherosclerosis which should be further explored.