Browsing by Author "Nordiana Pilus"

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    Digestive enzyme profiles from foregut contents of blue swimming crab, Portunus pelagicus from Straits of Johor, Malaysia
    (Journal of the Association of Arab Universities for Basic and Applied Sciences, 2016-10-17) Ambok-Bolong Abol-Munafi; Nordiana Pilus; Roswati Md Amin; Mohamad N. Azra; Mhd Ikhwanuddin
    This study describes the digestive enzyme profiles from foregut contents of the blue swimming crab, Portunus pelagicus (L.), from Straits of Johor, Malaysia. Adult male (mature and immature) and female (ovarian maturation stages 1–4) blue swimming crabs were assayed for the presence of digestive enzymes (protease, trypsin, amylase and lipase) in the foregut. The results show that in some instances, significant differences in enzyme activities were observed between the sexes of P. pelagicus. Protease, trypsin, and amylase were detected in all gonad maturation stages, suggesting that crabs including immature males can readily digest dietary protein and carbohydrates, but not lipids. Trypsin (except for ovarian maturation Stage 4) and lipase (ovarian maturation Stage 4 and mature male only) activities were considerably higher than amylase, indicating that dietary proteins and lipids are more important than carbohydrates and, thus, suggests a carnivorous diet. In conclusion, the changes in enzyme activities between the different maturation stages and sexes reflect natural changes in diet and feeding abilities of P. pelagicus.
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    Digestive enzyme profiles of forgut and their differential between sexes of blue swimming crab, portunus pelagicus at Gelang Patah
    (Universiti Malaysia Terengganu, 2014) Nordiana Pilus
    Blue swimming crab, Portunus pelagicus consumed variety kind of food as their diet. The study shows that their dietary items are molluscs, crabs and seagrass, including unidentified items that semi-digested. They are categorized as cannibalism because they consumed other crabs where the parts of crab's appendages were found in their foregut. They are omnivore group, as they eat other animal, they also consumed plants. Amylase, protease, and trypsin were detected in all sample and stages, except lipase, indicate that P. pelagicus readily digest dietary protein and carbohydrate at all stages. The changes in enzyme activities between stages reflect the changes in natural diet and feeding abilities. High proteolytic activity reflects a diet high in protein as show by matured males (MA). Meanwhile, carbohydrase activity reflects a diet with starch or cellulose that consumed by all stages especially matured males (MA), females with ovarian stage two (S2) and immature males (IM). And the high lipase activity reflects a diet high in fat as shows by female with ovarian stage four (S4).