Browsing by Author "Nor A. Kasan"

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    Isolation of bioflocculant-producing bacteria from Penaeus vannamei ponds for the production of extracellular polymeric substances
    (AACL Bioflux, 2016) Nor A. Kasan; Mohd F. Amin Che Teh; Nurul F. Che Hashim; Zaharah Ibrahim; Nakisah Mat Amin; Nurarina A. Ghazali
    Bioflocculants are essential polymers with their flocculating activity depending on the characteristics of the secreted flocculants by bioflocculant-producing bacteria. However, the characteristics of bioflocculant produced by microorganisms were not investigated profoundly. In order to better understand these characteristics, determination of extracellular polymeric substances (EPS) from bioflocculant-producing bacteria were characterized in terms of protein concentrations. A total of 51 bioflocculant-producing bacteria isolates were screened from Pacific whiteleg shrimp, Penaeus vannamei culture ponds in Setiu, Terengganu, Malaysia. Screening of bioflocculant-producing bacteria were conducted through morphological approaches followed by protein extraction using Lowry assay method. The identified bioflocculant-producing bacteria includes Corynebacterium sp., Klebsiella sp., Lactobacillussp., Staphylococcus sp., Bacillus sp., Streptococcus sp., Vibrio spp., Neisseria sp., Serratia sp. and Yersinia sp., with the highest protein concentration of 829 mg mL-1 were attained by Staphylococcus sp. The various amounts of EPS concentration produced by different species of bacteria were dependent on their specific population growth and growth rate. Therefore, the establishment of bioflocculant-producing bacteria isolated from biofloc which showed high tendency for EPS production were performed successfully.
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    Study on carbon sinks by classified biofloc phytoplankton from marine shrimp pond water
    (AACL Bioflux, 2016) Hidayah Manan; 1Julia H. Zhong Moh; Nor A. Kasan; Suhaimi Suratman; Mhd Ikhwanuddin
    Study on the carbon sink by biofloc phytoplankton from species Oocystis sp. (Chlorophyta) and Chroococcus sp. (Cyanophyta) was done from the study area of marine shrimp farm i- sharp Setiu, Terengganu. Three month of database for carbon sinks was collected from the selected phytoplankton in the pond. The biovolume of the phytoplankton was calculated using simple geometrical formula and carbon conversion factor to convert the biovolume of each class of phytoplankton to carbon sink amount of value. Overall, Chroococcus sp. was identified as the most abundance species found in the pond where it can sequestrate 14923.39 μ g C L-1 amount of carbon dioxide (CO2) as compared to the amount of carbon can be sequestrated by Oocystis sp. which is around 3778.20 μ g C L-1. It also can be claimed that Chroococcus sp. was more carbon dense compared to Oocystis sp. because of its bigger cell volume. It can be assumed that biofloc phytoplankton was successful in carbon sequestration and contributed as small part of carbon sinker in the world due to its ability to sequester CO2 and high abundances in number found in the pond make its more efficient in sinking carbon dioxide.