Browsing by Author "Lee Seong Wei"

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    Antibiotic and Heavy Metal Resistance of Aeromonas spp. Isolated from Diseased Red Hybrid Tilapia (Oreochromis sp.)
    (Annual Research Review in Biology, 2015-01) Lee Seong Wei
    A total of 300 bacterial isolates of Aeromonas spp. were isolated from diseased Red Hybrid Tilapia, Oreochromis sp. All the bacterial isolates were tested for antibiotic susceptibility against 16 types of antibiotics by using disk diffusion method. The antibiotics tested in this study were nalidixic acid (30 µg/disk), oxolinic acid (2 µg/disk), compound sulphonamides (300 µg/disk)
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    Characteristation of Edwardsiella tarda isolated from local Calrias gariepinus and potential remedies from edible plants
    (Fakulti Agroteknologi dan Sains Makanan, 2009-05) Lee Seong Wei
    This study characterised 7 isolates of Edwardsiella tarda from African catfish, Clarias gariepinus. The isolated E. tarda were identified using a combination of 29 conventional tests and commercial kit. Random amplification of polymorphic DNApolymerase chain reaction (RAPD PCR) analysis was done on these bacterial isolates using three universal primers, namely WTP, (GTG)s and M13 universal. Characterisation of whole cell protein profiles of the isolates were also carried out using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). In the present study, minimum inhibitory concentration (MIC) values of 20 edible plant extracts against E. tarda were determined through a two fold microdilution technique.