Browsing by Author "Arief Izzairy Zamani"

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    Bacterial flora associated with the giant fresh water prawn (macrobrachium rosenbergii) post-larvae in the hatchery system
    (Terengganu: Universiti Malaysia Terengganu, 2009) Arief Izzairy Zamani
    The objective of this study was to understand the bacterial flora associated with giant fresh water prawn, Macrobrachium rosenbergii (post-larvae) in University Malaysia Terengganu hatchery and it's resistant towards antibiotics and heavy metals tolerance. Total of 35 isolates were collected and determined their tolerance to antibiotics and heavy metal. The isolates comprised of 8 genera, Escherichia coli, Pseudomonas spp., Aeromonas spp., Vibrio spp., Shigella spp., Providencia spp., Enterobacter spp., and Klebsiella spp. which identified by morphological typing. Total plate counts ranged from 2.5x10 to 2.76 x 104 cfu m1-1 • The antibiotics test with 15 different antibiotics showed that all isolates were sensitive to Florfenicol (FFC). Multiple antibiotic resistance MAR index, 0.54 indicates that the isolates were originated from high-risk source of contamination. Heavy metals tolerance test such as Cu, Cr, Cd and Hg with 5 different concentrations showed tolerance pattern Hg = Cr > Cu > Cd. The study is very useful in contributing a preliminary data and profile of the bacteria associated with giant fresh water prawn, Macrobrachium rosenbergii (post-larvae).
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    Isolation and characterisation of heparin binding protein in slipper oyster, Crassostrea iredalei (faustino 1932) and its role in prophenoloxidase-activating system
    (Terengganu: Universiti Malaysia Terengganu, 2013-05) Arief Izzairy Zamani
    Recognition proteins play an important role in the immunodefense system of invertebrates. Thus, present study aimed to isolate a recognition protein, via Heparin column from Slipper cupped oyster, Crassostrea iredalei haemolymph plasma to elucidate its roles in prophenoloxidase-activation systems (proPO) and to characterise it. The protein was isolated using affinity column chromatography with yield of 8% and a single peak.