Browsing by Author "Amin Safwan Adnan"

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    Effect of water salinity on ovarian maturation stages and 17 b-estradiol levels on fermale orange mud crab, scylla olivacea (HERBST, 1796) in captivity
    (Universiti Malaysia Terengganu, 2016-10) Amin Safwan Adnan
    The present study was designed to focus on the effect of water salinity (10 ppt, 20 ppt and 30 ppt) on ovarian maturation stages and 17 β-estradiol levels on female orange mud crab, Scylla olivacea in captivity. Scylla olivacea were sampled from Setiu Wetlands, Terengganu on the East Coast of Peninsular Malaysia (5°31'23.1"N 102°55'56.1"E) from July to September 2015. Ovarian maturation stages were determined through colouration, Gonad Somatic Index (GSI), oocyte diameter and oocyte structure, all collected for analyzation at every 15 days throughout 60 days of culture period (Individual aquarium: 36cm x 22cm x 21 cm; Salinity: 10 ppt, 20 ppt, 30 ppt; Temperature: 27-29oC; Light intensity: ambient; Diet intake: Selaroides leptolepis at 10% body weight, every days, twice daily, at 0900 hrs and 1700 hrs), while the reproductive hormone levels 17 β-estradiol in the hemolymph were measured through Enzyme-Linked ImmunoSorbent Assay (ELISA). Morphologically, as the ovary develop, the colour start to change from translucent or whitish in colour and sometimes creamy to pale yellow, followed by light orange and lastly reddish orange.
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    Study on In-vitro Fertilization of Orange Mud Crab, Scylla olivvacea (Hebst, 1976)
    (Universiti Malaysia Terengganu, 2014) Amin Safwan Adnan
    Treated with eyestalk ablation and continuous feeding of squids which is Loligo spp, female mud crab, Scylla olivacea were induced for ovarian maturation till Stage 4 of ovary maturation stage in order to perform In-vitro trials. After the female crabs were fully mature enough Stage 4, the crabs were selected and been dissected. There are eight trials that have been done and the trials were divided into two parts which are by using sperms in spermatheca of female crabs and the other one is by using sperms in vas deferens of male crabs. Stages 4 ovary was combined with sperms and were maintained in small aquarium with 30 ° C of water temperature set by using water heater. Daily observation was done by using compound and advance microscope while the fertilization rate and early stage of embryonic development were calculated and being identified. The fertilization rates were different in each trial. Trial 1, 2 and 3 were failed and starting from Trial 4 until Trial 8, the fertilization rate starting to give favorable result.