Browsing by Author "AHMAD SHAMSUDDIN AHMAD"

Now showing 1 - 2 of 2
  • Thumbnail Image
    Item
    THE POTENTIAL OF CAROTENOIDS FROM MARINE TROPICAL MICROALGAE IN THE HEALING PROCESS OF GASTRITIS
    (Journal of Sustainability Science and Management, 2015) AHMAD SHAMSUDDIN AHMAD; YONG JULIUS FU SIONG*; DESY FITRYA SYAMSUMIR; NOR ATIKAH MOHAMED ZIN; SITI AISHA MOHD RADZI; MURNI NUR ISLAMIAH KASSIM; MOHD ARIFF MUZAMEL; MOHD RIDZUAN YUSOF; THIRUKANTHAN CHANDRA SEGARAN
    Carotenoids which are important towards the biological activities are produced by all higher plants, some bacteria and algae. Marine microalgae can produce a variety of substances including carotenoids and it has a commercial interest as production of microalgae in controlled condition is affordable. Three species of marine microalgae; Chlorella sp., Dunaliella sp. and Isochrysis sp. were cultured and carotenoids were extracted for testing. The total carotenoid present in dry weight of Chlorella sp., Dunaliella sp. and Isochrysis sp. were 55.1%, 99.3% and 29.4% respectively. The carotenoids from the microalgae were tested for toxicity level against MCF7 cancer cells, with Chlorella sp., Dunaliella sp. and Isochrysis sp. showing IC50 value 0.25mg/ml, 0.3 mg/ml and 0.32 mg/ml from crude extract respectively. Antioxidant di(phenyl)-(2,4,6-trinitrophenyl)iminoazanium (DPPH) scavenging activity were present in all the three types of microalgae. In-vivo gastritis studies on model mice showed healed result from Dunaliella sp. but not from Chlorella sp. and Isochrysis sp. From the three microalgae species, Dunaliella sp. has the highest ability to promote gastric healing as it contains antioxidant and antimicrobial properties.
  • Thumbnail Image
    Item
    TAXONOMICAL IDENTITY AND POLYSACCHARIDE PRODUCED BY Bacillus SPECIES ISOLATED FROM OLD AGED MEDICINAL DECOCTIONS
    (Journal of Sustainability Science and Management, 2011) TEVAN RAMANATHAN; AZIZ AHMAD; AHMAD SHAMSUDDIN AHMAD; MURUGAN KALIMUTHO
    In Malay folk medicine, bacterial broth are used in treating diseases such as sore throat and fever. However, the characterisation and identifcation of the bacterial are not know. In the present study, the bacterial broth used as a decoction in Malay folk medicine was investigated. The polysaccharide-producing bacteria were isolated and characterised based on a series of morphological and biochemical assays. A total of 14 isolates tentatively identifed as Bacillus spp. were further characterised using a PIBWin bacterial identifcation program. The identifcation revealed that the cocktail of bacteria belongs to species of Bacillus subtilis ssp. subtilis, Bacillus pumilis, Bacillus coagulans and Bacillus lincheniformis. Five out of 14 isolates, B. coagulans, B. subtilis ssp. subtilis, B. lincheniformis, and two Bacillus spp. were found to produce exo-polysaccharide under static culture conditions. B. coagulans produced the highest amount of polysaccharide followed by B. lincheniformis and B. subtilis ssp. Subtilis. Types of monosaccharide were tentatively identifed using paper chromatography. B. coagulans possesses almost all types of monosaccharides, whereas in the other strains, lactose, raffnose, galactose, glucose, mannose, xylose and/or rhamnose were identifed. The existence of monosaccharide between these isolates showed that the exopolysaccharide produced by Bacillus spp. could possibly be utilised for medicinal purposes. In addition, the identifed bacterial polysaccharide may also have a role in anti-bacterial responses to the traditional treatment approach.