Optimization of protein enzymatic hydrolysis of blood cockle (Anadara granosa)

Abstract

The objective of this study is to establish the optimum hydrolysis conditions for blood cockle cockles (Anadara granosa) using Alcalase® by employing response surface methodology (RSM). A three-level face-centered central composite design (CCD) was adapted in the study. The effects of temperature, enzyme to substrate level (EIS % ) and hydrolysis time were studied in order to obtain the maximum degree hydrolysis (DH%). The optimal condition for protein enzymatic hydrolysis of cockle were found to be at 65 C for temperature, pH at 9 .5 enzyme concentration at 2% and hydrolysis time of 180 minutes. The maximum DH obtained was 37.27%, meanwhile the predicted values for maximum DH was 34.04% for cockle hydrolysis. The enzymatic hydrolysis of cockles gave a quadratic fit with the experimental data. The proximate composition of the lyophilized cockle hydrolysate contained 8.59% moisture, 74.00% protein content, 5.80% fat, 10.22% ash and 1.39% carbohydrate.