Please use this identifier to cite or link to this item: http://umt-ir.umt.edu.my:8080/handle/123456789/9352
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dc.contributor.authorFara Nor Asmira Jastan-
dc.date.accessioned2018-07-18T03:01:37Z-
dc.date.available2018-07-18T03:01:37Z-
dc.date.issued2007-
dc.identifier.urihttp://umt-ir.umt.edu.my:8080/xmlui/handle/123456789/9352-
dc.description.abstractA study was done to determine the gene expression profiling in Cf arias batrachus fed with omega-3 enriched and non omega-3 enriched pellet. This study involves several steps beginning with sample collection, followed by RNA extraction and purification, RNA quantification, gene expression profiling using (DD) RT-PCR technique, analysis of gene expression profile and isolation of expressed genes. A total of 5 samples of Asian catfish, Clarias batrachus fed with Omega-3 enriched pellet non omega-3 enriched pellet were collected. The results of the experiment showed that there was a difference in the gene expression profiling between C.batrachus fed with omega-3 enriched and non omega-3 enriched pellet when amplified using Arbitrary Primer ACP2, ACP5, ACP7, ACP 11, ACP 17 and ACP20. Range of DNA fragments obtained from the experiment were within 1 OObp to 1300bp. Overall observation found that several genes have been down-regulated in C.batrachus fed with omega-3 enriched pellet as it contained less DNA fragments compared to C.batrachus fed with non omega-3 enriched pellet.en_US
dc.language.isoenen_US
dc.publisherUniversiti Malaysia Terengganuen_US
dc.subjectFara Nor Asmira Jastanen_US
dc.subjectLP 7 FASM 2 2007en_US
dc.titleGene expression profiling of Clarias batrachus FED with omega-3 enriched pellet and non omega-3 enriched pelleten_US
dc.typeWorking Paperen_US
Appears in Collections:Fakulti Agroteknologi dan Sains Makanan

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