Please use this identifier to cite or link to this item: http://umt-ir.umt.edu.my:8080/handle/123456789/9352
Title: Gene expression profiling of Clarias batrachus FED with omega-3 enriched pellet and non omega-3 enriched pellet
Authors: Fara Nor Asmira Jastan
Keywords: Fara Nor Asmira Jastan
LP 7 FASM 2 2007
Issue Date: 2007
Publisher: Universiti Malaysia Terengganu
Abstract: A study was done to determine the gene expression profiling in Cf arias batrachus fed with omega-3 enriched and non omega-3 enriched pellet. This study involves several steps beginning with sample collection, followed by RNA extraction and purification, RNA quantification, gene expression profiling using (DD) RT-PCR technique, analysis of gene expression profile and isolation of expressed genes. A total of 5 samples of Asian catfish, Clarias batrachus fed with Omega-3 enriched pellet non omega-3 enriched pellet were collected. The results of the experiment showed that there was a difference in the gene expression profiling between C.batrachus fed with omega-3 enriched and non omega-3 enriched pellet when amplified using Arbitrary Primer ACP2, ACP5, ACP7, ACP 11, ACP 17 and ACP20. Range of DNA fragments obtained from the experiment were within 1 OObp to 1300bp. Overall observation found that several genes have been down-regulated in C.batrachus fed with omega-3 enriched pellet as it contained less DNA fragments compared to C.batrachus fed with non omega-3 enriched pellet.
URI: http://umt-ir.umt.edu.my:8080/xmlui/handle/123456789/9352
Appears in Collections:Fakulti Agroteknologi dan Sains Makanan

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