Please use this identifier to cite or link to this item: http://umt-ir.umt.edu.my:8080/handle/123456789/9350
Full metadata record
DC FieldValueLanguage
dc.contributor.authorNoor Shazana Mohd Shamlan-
dc.date.accessioned2018-07-18T03:01:21Z-
dc.date.available2018-07-18T03:01:21Z-
dc.date.issued2007-
dc.identifier.urihttp://umt-ir.umt.edu.my:8080/xmlui/handle/123456789/9350-
dc.description.abstractA study was done to develop a Nested PCR protocol for the isolation of partial growth hormone (GH) gene fragment of Marble Goby. In this study, DNA from Marble Goby (Oxyeleotris marmorata) was extracted from the muscle tissue and used for PCR amplification. In the nested PCR protocol, amplification was done using two pairs of degenerate primers that were specific to the GH gene sequence. In this experiment, Nested PCR was used to confirm the PCR product as it allows discrimination between specific and nonspecific amplification signals. In this experiment, several optimizations were done. The optimizations involved four parameters that are annealing temperature, PCR cycle, concentration of Magnesium Chloride (MgCh) and polymerase enzymes. From the results obtained, the optimum annealing temperature was 32 ° C, the optimum PCR cycle was 35, the optimum MgCh concentration was 2mM and the optimum enzymes used was Finnzymes DyNAzyme EXT DNA polymerase.en_US
dc.language.isoenen_US
dc.publisherUniversiti Malaysia Terengganuen_US
dc.subjectNoor Shazana Mohd Shamlanen_US
dc.subjectLP 13 FASM 2 2007en_US
dc.titleDevelopment of nested PCR protocol for the isolation of partial growth hormone gene fragment in marble gobyen_US
dc.typeWorking Paperen_US
Appears in Collections:Fakulti Agroteknologi dan Sains Makanan

Files in This Item:
File Description SizeFormat 
LP 13 FASM 2 2007 Abstract.pdf485.53 kBAdobe PDFView/Open
LP 13 FASM 2 2007 Full text.pdf
  Restricted Access
2.47 MBAdobe PDFView/Open Request a copy


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.