Effects oral vaccination of killed Pasteurella multocida B:2 on bronchus-associated lymphoid tissue (BALT) in white rats

Abstract

Pasteurella multocida is a coccobacillus bacterium that has been recognized as an important animal pathogen and this type of bacteria always exists in the respiratory tract of different animals. Thus, this study was conducted to determine the effects oral vaccination of killed Pasteurella multocida B:2 on Bronchus-associated lymphoid tissue (BAL T). This study involved with forty-two clinically healthy rats (Rattus novegicus) from type Sprague- Dawley that were randomly allocated into two groups. Each group contains 21 rats for the 7 weeks experiment duration. Group 1 was the control untreated group while Group 2 was the treated group. The treated groups were exposed twice with oral vaccination with two week interval and every exposure consists 15g vaccinated pellet with killed Pasteurella multocida B:2 for each rat. Then, every week on a designated day, three white rats from the each group were slaughtered to collect the rat's lung samples throughout project for seven weeks. After that, histological works was done to all lung samples to observe bronchus-associated lymphoid tissue (BAL T). The results of the study using oral vaccination on the area of BAL T and the number of lymphocytes show a very positive response having (p< 0.01) between the control and the treatment group. The data analysis was conducted using the ANOV A Two-Factor with Replication. The response between the weeks also shows a significant difference having (p< 0.05) in both area ofBALT and the number of lymphocytes. Results obtained proved that the BAL T response well and the oral vaccination manage to induce immunity towards the Pasteurella multocida B:2 in rats.