Genotoxic effect of two Acanthamoeba lysates on Hela cells

Abstract

Genotoxic effect of Acanthamoeba lysates was studied on human cervical cancer cell line. Lysates were obtained from Acanthamoeba polyphaga which are freshwater isolate and Acanthamoeba (HKL isolate) which is a clinical isolation from corneal scrapings of keratitis patient. These lysates were treated on HeLa cells by using MTT assay. The mitochondrial dehydrogenases in living cells reduced the MTT substances to a blue-magenta colored formazan crystals. The number of intact alive cells was directly proportional to the amount of crystal formed. Results obtained from the study indicated that the concentration of lysates that inhibited 50% (IC50) of the cancer cells population for Acanthamoeba polyphaga and Acanthamoeba (HKL isolate) lysates are 85.0 µg/mL and 3.0 µg/mL, respectively while the IC25 values are 42.5 µg/mL and 1.5 µg/mL, respectively. The genotoxic effect of these lysates on HeLa cells by their IC50 and IC25 values were studied by using Comet assay. From the DNA damage observed, Acanthamoeba (HKL isolate) lysate showed severe genotoxic effect compared to the lysate of Acanthamoeba polyphaga.