Please use this identifier to cite or link to this item: http://umt-ir.umt.edu.my:8080/handle/123456789/8483
Title: Electroporation of Chlorella sp. with pCAMBIA 1304 circular construct
Authors: Yeong Yun Yee
Keywords: Yeong Yun Yee
LP 76 FST 2 2007
Issue Date: 2007
Publisher: Terengganu: Universiti Malaysia Terengganu
Abstract: An efficient method for electrically introducing pCAMBIA 1304 circular plasmid into Chlorella cells was developed. This method is useful for molecular genetic studies and commercial applications where the genes of interest responsible for the production of a valuable nutrient are electrically introduced into the recombinant Chlorella sp. The pCAMBIA 1304 circular plasmid carrying the hygromycin phosphotransferase (hpt) selectable marker, green fluorescent protein (gfp) and �­ glucuronidase (gus) reporter genes regulated by CaMV 35S promoter was used to transform the Chlorella cells. The pCAMBIA 1304 circular plasmid was successfully extracted from E. coli and verified by PCR technique with three sets of primers combination.
URI: http://hdl.handle.net/123456789/8483
Appears in Collections:Fakulti Sains dan Teknologi

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