Please use this identifier to cite or link to this item: http://umt-ir.umt.edu.my:8080/handle/123456789/8469
Title: Cloning and sequencing of putative anhydrotetracycline oxygenase (Ahtm) gene from amoeba (Acanthamoeba spp.)
Authors: Saw Ying Yu
Keywords: Saw Ying Yu
LP 62 FST 2 2007
Issue Date: 2007
Publisher: Terengganu: Universiti Malaysia Terengganu
Abstract: Acanthamoeba spp. was believed to possess vanous antimicrobial peptides and proteins to combat bacterial growth inside their phagosomes. Tetracycline belongs to a group of antibiotics that contain antimicrobial and pharmacokinetic properties. Anhydrotetracycline oxygenase (anhydrotetracycline monooxygenase or AHTM) is the enzyme that involve in the reaction of biosynthesis pathway of Streptomyces spp. in the production of tetracycline. Previous studies had successfully identified six putative fragments by using PCR technique. The putative fragment of AHTM5 was selected and re-amplified and cloned into pGEM-T Easy Vector. The putative recombinant colonies were selected for plasmid extraction. The presence of inserted DNA was confirmed by using PCR technique. The complete nucleotide sequence consists of 243bp while the deduced amino acids from the complete nucleotide sequence are 81. The translated amino acid sequence of clone pAHTM5 shows 81 %85%
URI: http://hdl.handle.net/123456789/8469
Appears in Collections:Fakulti Sains dan Teknologi

Files in This Item:
File Description SizeFormat 
LP 62FST 2 2007 Abstract.pdf829.41 kBAdobe PDFView/Open
LP 62FST 2 2007 Full Text.pdf
  Restricted Access
2.49 MBAdobe PDFView/Open Request a copy


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.