Please use this identifier to cite or link to this item: http://umt-ir.umt.edu.my:8080/handle/123456789/8445
Full metadata record
DC FieldValueLanguage
dc.contributor.authorNorbaiti Mohd Isa-
dc.date.accessioned2018-02-12T04:02:01Z-
dc.date.available2018-02-12T04:02:01Z-
dc.date.issued2007-
dc.identifier.urihttp://hdl.handle.net/123456789/8445-
dc.description.abstractElectroporation has been used in plants and microalgae genetic engmeenng to produce beneficial transgenic plants and microalgae. Genetic transformation by electroporation increase the level of polyunsaturated fatty acids in Ch/ore/la sp. such as m-3 C18:3 and m-6 C18:2. The objectives of this study are to transform Ch/ore/la sp. with p35S-AP construct by electroporatian and to select for the putative recombinant chlorella cells. The extracted plasmid DNA of p35S-AP was verified using PCR technique. In this step, three types of primer combination were used as follows: 35S-F/35S-R, PTE-VF1/PTE-VR2 and 35S-F/PTE-VF1. The 35S-F/35S-R primer combination successfully amplified the CaMV 35S promoter with the size of 326 bp. The combination of PTE-VF1/PTE-VR2 primer successfully amplified the antisense palmitoyl-ACP thioesterase gene fragment with the size of 617 bp while 35S-F/PTE-VF1 successfully amplified the CaMV 35S promoter and antisense palmitoyl-ACP thioesterase gene fragment with the size of 934 bp. The DNA plasmid was then digested with EcoRl restriction enzyme to produce linear plasmid followed by DNA plasmid purification.en_US
dc.language.isoenen_US
dc.publisherTerengganu: Universiti Malaysia Terengganuen_US
dc.subjectNorbaiti Mohd Isaen_US
dc.subjectLP 38 FST 2 2007en_US
dc.titleElectroporation of Chlorella sp. with p35s-AP linearized constructen_US
dc.typeWorking Paperen_US
Appears in Collections:Fakulti Sains dan Teknologi

Files in This Item:
File Description SizeFormat 
LP 38 FST 2 2007 Abstract.pdf814.7 kBAdobe PDFView/Open
LP 38 FST 2 2007 Full Text.pdf
  Restricted Access
2.7 MBAdobe PDFView/Open Request a copy


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.