Please use this identifier to cite or link to this item: http://umt-ir.umt.edu.my:8080/handle/123456789/8445
Title: Electroporation of Chlorella sp. with p35s-AP linearized construct
Authors: Norbaiti Mohd Isa
Keywords: Norbaiti Mohd Isa
LP 38 FST 2 2007
Issue Date: 2007
Publisher: Terengganu: Universiti Malaysia Terengganu
Abstract: Electroporation has been used in plants and microalgae genetic engmeenng to produce beneficial transgenic plants and microalgae. Genetic transformation by electroporation increase the level of polyunsaturated fatty acids in Ch/ore/la sp. such as m-3 C18:3 and m-6 C18:2. The objectives of this study are to transform Ch/ore/la sp. with p35S-AP construct by electroporatian and to select for the putative recombinant chlorella cells. The extracted plasmid DNA of p35S-AP was verified using PCR technique. In this step, three types of primer combination were used as follows: 35S-F/35S-R, PTE-VF1/PTE-VR2 and 35S-F/PTE-VF1. The 35S-F/35S-R primer combination successfully amplified the CaMV 35S promoter with the size of 326 bp. The combination of PTE-VF1/PTE-VR2 primer successfully amplified the antisense palmitoyl-ACP thioesterase gene fragment with the size of 617 bp while 35S-F/PTE-VF1 successfully amplified the CaMV 35S promoter and antisense palmitoyl-ACP thioesterase gene fragment with the size of 934 bp. The DNA plasmid was then digested with EcoRl restriction enzyme to produce linear plasmid followed by DNA plasmid purification.
URI: http://hdl.handle.net/123456789/8445
Appears in Collections:Fakulti Sains dan Teknologi

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