Establishment tissue culture of Bruguiera sexangula

Abstract

The objectives of this study is to determine the most effective method for surface sterilization, the most suitable media for regeneration and hence to establish a tissue culture technique of Bruguiera sexangula. This is because this technology can produce a large number of parent genetically same plants rapidly. In same time, B. sexangula is special for having many uses as anti-tumor and produce tannin for leather industry. For this study, explants in form of shoot tips, fruits and seedlings obtained from mangrove area near Universiti Malaysia Terengganu were immersed for various time periods and various concentration of commercial bleach (Clorox) in searching for best surface· sterilization method. For tissue culture establishment, Murashige and Skoog (1962) and X media was used. It is found that, the best method for surface sterilization is immersed shoot tips, fruits and seedlings in 90% Clorox for l O minutes, 60% for l O minutes and 80% for 10 minutes respectively. Prolong the time was found to damage explants tissue. However, B. sexangula was not truly successful cultured due to browning problem and high degree of contamination.