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dc.contributor.authorLua Chung Liang-
dc.date.accessioned2018-02-11T08:16:46Z-
dc.date.available2018-02-11T08:16:46Z-
dc.date.issued2007-
dc.identifier.urihttp://hdl.handle.net/123456789/8422-
dc.description.abstractElectroporation is an important genetic transformation tool that has been used in plant genetic engineering to generate a wide variety of fertile transgenic plants. The PSP' AP-VF2 construct carries antisense palmitoyl-ACP thioesterase cDNA driven by Sesquiterpene synthase promoter. The AP2 construct was isolated from E.coli. The purity of the DNA obtained was 1.81 and its concentration was 0.15 µg/µL. The PSP' AP-VF2 plasmid was verified by PCR technique with primer combinations of PTE-VF1/PTE-VR2 and PTE-VF1/Pro-VF2. The size of amplified bands were 617 bp and 1047 bp respectively. The desired plasmid was successfully linearized by using EcoRl restriction enzymes and verified by 1.0% agarose gel electrophoresis. Purified linearized product shows the purity of 1.91 and its concentration was 0.40 µg/µL. Different parameters were used to determine the suitable voltage for transformation using p35S-AP. Agr mode (2.2kV) shows the growth of Ch/ore/la sp.en_US
dc.language.isoenen_US
dc.publisherTerengganu: Universiti Malaysia Terengganuen_US
dc.subjectLP 27 FST 2 2007en_US
dc.subjectLua Chung Liangen_US
dc.titleElectroporation of Chlorella sp. with linearized psp AP-VF2 constructen_US
dc.typeWorking Paperen_US
Appears in Collections:Fakulti Sains dan Teknologi

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