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http://umt-ir.umt.edu.my:8080/handle/123456789/8422
Title: | Electroporation of Chlorella sp. with linearized psp AP-VF2 construct |
Authors: | Lua Chung Liang |
Keywords: | LP 27 FST 2 2007 Lua Chung Liang |
Issue Date: | 2007 |
Publisher: | Terengganu: Universiti Malaysia Terengganu |
Abstract: | Electroporation is an important genetic transformation tool that has been used in plant genetic engineering to generate a wide variety of fertile transgenic plants. The PSP' AP-VF2 construct carries antisense palmitoyl-ACP thioesterase cDNA driven by Sesquiterpene synthase promoter. The AP2 construct was isolated from E.coli. The purity of the DNA obtained was 1.81 and its concentration was 0.15 µg/µL. The PSP' AP-VF2 plasmid was verified by PCR technique with primer combinations of PTE-VF1/PTE-VR2 and PTE-VF1/Pro-VF2. The size of amplified bands were 617 bp and 1047 bp respectively. The desired plasmid was successfully linearized by using EcoRl restriction enzymes and verified by 1.0% agarose gel electrophoresis. Purified linearized product shows the purity of 1.91 and its concentration was 0.40 µg/µL. Different parameters were used to determine the suitable voltage for transformation using p35S-AP. Agr mode (2.2kV) shows the growth of Ch/ore/la sp. |
URI: | http://hdl.handle.net/123456789/8422 |
Appears in Collections: | Fakulti Sains dan Teknologi |
Files in This Item:
File | Description | Size | Format | |
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LP 27 FST 2 2007 Abstract.pdf | 744.92 kB | Adobe PDF | View/Open | |
LP 27 FST 2 2007 Full Text.pdf Restricted Access | 2.86 MB | Adobe PDF | View/Open Request a copy |
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