Please use this identifier to cite or link to this item: http://umt-ir.umt.edu.my:8080/handle/123456789/8421
Title: Cloning and sequencing of putative B-Lactam gene from selected amoeba isolate
Authors: Lim Wei Chun
Keywords: LP 26 FST 2 2007
Lim Wei Chun
Issue Date: 2007
Publisher: Terengganu: Universiti Malaysia Terengganu
Abstract: Penicillin is useful for the treatment of bacterial infections. Microorganism such as Acanthamoeba castelanii that inhibit the biosynthesis of the bacterial cell wall are believe to produce penicillin antibiotics. Previous research by Chuah Huey Shuan had identified twelve specific bands using primer combinations of IPNS-Fl/IPNS-R2, IPNS-F2/IPNS-R2 and IPNS-F2/IPNS-RI with gradient PCR technique from ten isolates of amoeba. The MAI fragment (850 bp) was selected for cloning, sequencing and analyzing. The MAI fragment was reamplified using combination of IPNSĀ­ FI/IPNS-R2 primer with PCR technique.
URI: http://hdl.handle.net/123456789/8421
Appears in Collections:Fakulti Sains dan Teknologi

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