Electrotransformation of Agrobacterium Tumefaciens with p 35S-AP and pCambia 1304 constructs

Please use this identifier to cite or link to this item: http://umt-ir.umt.edu.my:8080/handle/123456789/7965

Title:	Electrotransformation of Agrobacterium Tumefaciens with p 35S-AP and pCambia 1304 constructs
Authors:	Tan Lay Kim
Keywords:	Tan Lay Kim LP 60 FST 3 2006
Issue Date:	2006
Publisher:	Terengganu: Kolej Universiti Sains dan Teknologi Malaysia
Abstract:	Agrobacterium-mediated gene transfer is an important genetic transformation tool that has been used in plant genetic engineering to generate a wide variety of fertile transgenic plants. The p35S-AP construct carries antisense palmitoyl-ACP thioesterase cDNA driven by CaMV 35S promoter while the pCAMBIA 1304 construct carries genes encoding p-glucuronidase (uidA) and green fluorescent protein (gfp ). Both constructs were successfully electroporated into wild type A. tumefaciens strain LBA 4404 using electroporation apparatus, MicroPulser.
URI:	http://hdl.handle.net/123456789/7965
Appears in Collections:	Fakulti Sains dan Teknologi

Files in This Item:

File	Description	Size	Format
LP 60 FST 3 2006 Abstract.pdf		600.81 kB	Adobe PDF	View/Open
LP 60 FST 3 2006 Full Text.pdf Restricted Access		3.86 MB	Adobe PDF	View/Open Request a copy

DSpace JSPUI