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http://umt-ir.umt.edu.my:8080/handle/123456789/6493
Title: | Cryostorage of cryptocoryne ciliata |
Authors: | Chee Pui Yee |
Keywords: | LP 4 FST 1 2005 Chee Pui Yee |
Issue Date: | 2005 |
Publisher: | Terengganu: Kolej Universiti Sains dan Teknologi Malaysia |
Abstract: | Twenty pnmers were screened. Four pnmers, OPA4, OPA7, OPAlO and OPA16 showed the clearest bands and polymorphism was choosen for C,yptocoryne ciliata. Vitrification technique was used to obtain the cold storage procedure for C. ciliata. No surviving explant was obtained after being immersed in liquid nitrogen. Preculture, loading, dehydration, unloading and warming upon cold storage of explants were examined. After two to four days of preculture, 30 minutes of loading period, one hour of dehydration in PVS (Plant Vitrification Solution), 20 minutes of unloading treatment and warming in 40 °C for one minutes. |
URI: | http://hdl.handle.net/123456789/6493 |
Appears in Collections: | Fakulti Sains dan Teknologi |
Files in This Item:
File | Description | Size | Format | |
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LP 4 FST 1 2005 Abstract.pdf | 555.89 kB | Adobe PDF | View/Open | |
LP 4 FST 1 2005 Full Text.pdf Restricted Access | 3.36 MB | Adobe PDF | View/Open Request a copy |
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