The effect of auxin on in vitro cultures of Cryptocoryne ciliata

Abstract

A study was conducted mainly to determine the effect of auxin on in vitro cultures of Cryptocoryne ciliata. The growth (fresh and dry weight), reducing sugar, and total soluble protein were determined after 30 days of treatment. The addition of different types of auxin with different concentrations induced root formation clifferentially. The type of auxin used were indole acetic acid (IAA), naphthalene acetic acid (NAA) and indole butyric acid (IBA). The protein content was low for high levels of concentration of auxin IAA and IBA (0.6 mg/I and 0.8 mg/I). The reducing sugar content was high without the presence of benzylaminopurine (BAP) in the medium. The suitable auxin for root induction was 0.5 mg/I IBA (with BAP) and in the absence of benzylaminopurine (BAP), the preferred auxin was IAA with 1.0 mg/I concentration.