Please use this identifier to cite or link to this item: http://umt-ir.umt.edu.my:8080/handle/123456789/5871
Title: Direct Shoot Regeneration from Callus of Melicope lunu-ankenda
Authors: Ab. Zuraida Rahman
Ayu Nazreena Othman
Fatin Liyana Izzati Kamaruddin
Aziz Bin Ahmad
Keywords: Melicope lunu-ankenda
Regeneration
Plant Growth Regulator
In Vitro
Issue Date: Feb-2015
Publisher: Natural Science
Abstract: Melicope lunu-ankenda is commonly used in traditional medicine. The conventional propagation method for this species is inefficient due to low propagation rate and its lengthy period to maturity. In addition, insufficient planting materials often pose a problem for the plantation sector. The tissue culture technique is best alternative to overcome the problems. The callus induction and direct shoot regeneration protocols for M. lunu-ankenda were established. Callus was successfully initiated from leaves explants cultured in MS medium added with 2,4-D at concentrations 0.5 to 5.0 mg/L singly or in combination with NAA at concentrations 1.0 to 10 mg/L. Shoot was regenerated from callus in phytohormone-free medium, BAP at concentrations 0.5 - 5.0 mg/L singly or in combination of BAP with NAA or 2,4-D at concentration 0.5 and 1.0 mg/L, respectively. BAP at 1.0 mg/L induced the highest shoot regeneration rate (80%) and number of plantlet per calli. The established methods might be used for production of phytochemicals and plantlets in large scale.
URI: http://hdl.handle.net/123456789/5871
Appears in Collections:Journal Articles

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