Please use this identifier to cite or link to this item: http://umt-ir.umt.edu.my:8080/handle/123456789/5671
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dc.contributor.authorS.N. Fatihah-
dc.contributor.authorS. Jasmani-
dc.contributor.authorA.B. Abol-Munafi-
dc.contributor.authorS. Noorbaiduri-
dc.contributor.authorH. Muhd-Farouk-
dc.contributor.authorM. Ikhwanuddin-
dc.date.accessioned2017-04-11T03:26:28Z-
dc.date.available2017-04-11T03:26:28Z-
dc.date.issued2016-
dc.identifier.citationVol.462; 56-63 p.en_US
dc.identifier.issn448486-
dc.identifier.urihttp://hdl.handle.net/123456789/5671-
dc.description.abstractThis study aimed to develop a cryopreservation protocol for spermof themud spiny lobster, Panulirus polyphagus. Spermof P. polyphaguswere successfully cryopreserved using a protocolwith cooling periods of 15min per temperature, 25, 20, 16, 4, 2, −4, −20, −80, and −150 °C, followed by immediate storage in liquid nitrogen (at −196 °C). The efficacy of the cryopreserved protocolwas determined by assessing the viability of sperm. The optimal thawing temperature for cryopreservation of sperm was 26 °C for 30 s, with a viability rate of 76.09% ± 7.81. At room temperature, −20 and −80 °C, 10% glycine provided the highest percentage of sperm viability at 91.87 ± 2.03% (5 min at room temperature), 91.31 ± 2.65% (6 h at −20 °C) and 75.88 ± 10.81% (6 h at −80 °C). In conclusion, we developed a protocol (Protocol I) for the successful cryopreservation of P. polyphagus sperm using Ca-F saline as an extender and 10% glycine as a cryoprotectant.en_US
dc.language.isoenen_US
dc.publisherAquacultureen_US
dc.subjectCryoprotectanten_US
dc.subjectExtenderen_US
dc.subjectLiquid nitrogenen_US
dc.subjectCooling ratesen_US
dc.subjectThawing temperatureen_US
dc.subjectCrustaceanen_US
dc.titleDevelopment of a sperm cryopreservation protocol for the mud spiny lobster, Panulirus polyphagusen_US
dc.typeArticleen_US
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