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Title: | Development of a sperm cryopreservation protocol for the mud spiny lobster, Panulirus polyphagus |
Authors: | S.N. Fatihah S. Jasmani A.B. Abol-Munafi S. Noorbaiduri H. Muhd-Farouk M. Ikhwanuddin |
Keywords: | Cryoprotectant Extender Liquid nitrogen Cooling rates Thawing temperature Crustacean |
Issue Date: | 2016 |
Publisher: | Aquaculture |
Citation: | Vol.462; 56-63 p. |
Abstract: | This study aimed to develop a cryopreservation protocol for spermof themud spiny lobster, Panulirus polyphagus. Spermof P. polyphaguswere successfully cryopreserved using a protocolwith cooling periods of 15min per temperature, 25, 20, 16, 4, 2, −4, −20, −80, and −150 °C, followed by immediate storage in liquid nitrogen (at −196 °C). The efficacy of the cryopreserved protocolwas determined by assessing the viability of sperm. The optimal thawing temperature for cryopreservation of sperm was 26 °C for 30 s, with a viability rate of 76.09% ± 7.81. At room temperature, −20 and −80 °C, 10% glycine provided the highest percentage of sperm viability at 91.87 ± 2.03% (5 min at room temperature), 91.31 ± 2.65% (6 h at −20 °C) and 75.88 ± 10.81% (6 h at −80 °C). In conclusion, we developed a protocol (Protocol I) for the successful cryopreservation of P. polyphagus sperm using Ca-F saline as an extender and 10% glycine as a cryoprotectant. |
URI: | http://hdl.handle.net/123456789/5671 |
ISSN: | 448486 |
Appears in Collections: | Journal Articles |
Files in This Item:
File | Description | Size | Format | |
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J2016-59-Development of a sperm cryopreservation protocol for the mud spiny lobster, Panulirus polyphagus.pdf | Full text | 492.33 kB | Adobe PDF | View/Open |
59-Development_of_a_sperm_cryopreservation_protocol_for_the_mud_spiny_lobster,_Panulirus_polyphagus.png | Evidence | 122.5 kB | image/png | View/Open |
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