A study on the genetic variation, cross compatibility and larval rearing of gouramis (trichogaster spp.)

Abstract

Genetic variation of four species of gouramis from the genus Trichogaster namely T. trichoptems (three spot gourami), T. leeri (pearl gourami), T. pectoralis (snakeskin gourami) and T. microlepis (moonlight gourami) and three varieties of three spot gouramis namely yellow gourami, cosby gourami and lavender gourami were determined using Random Amplified Polymorphic DNA (RAPD) techniques. A total of seven individuals from each species and varieties were used for RAPD assay using two different primers namely OPA-11 and OPA-12. No polymorphism was detected in blue, lavender and cosby gouramis. The genetic distance between seven pearl gourami individuals were ranged from 0.0000 to 0.0370. The genetic distance obtained from moonlight gourami and snakeskin gourami ranged from 0.0000 to 0.4545 and 0.0000 to 0.2727, respectively.