IN VITRO AND IN VIVO ANTI-INFLAMMATORY ACTIVITIES OF Hibiscus tiliaceus LEAVES EXTRACT

Abstract

Inflammation has become the focus of global scientific research because of its implication in virtually all human and animal diseases. The anti-inflammatory drugs such as corticosteroids and non-steroidal anti-inflammatory drugs (NSAID) have their side effects. Therefore, the anti-inflammatory drugs from the natural product had been researched in this study. This study aimed to profile and determines the chemical constituents of H. tiliaceus leaves extract and fractions as well to determine the anti-inflammatory activities of methanol extract of Hibiscus tiliaceus leaves (HM) in vitro on lipopolysaccharide (LPS) stimulated RAW264.7 cell lines and in vivo using formalin-induced paw eodema. HM was obtained by extraction process. Further fractionation of HM was done by using solvent-solvent partitioning and yielded four solvent fractions; hexane fraction (HH), dichloromethane fraction (HD), ethyl acetate fraction (HE) and water fraction (HW) of Hibiscus tiliaceus leaves. Antioxidant potency of HM and H. tiliaceus leaves fractions was tested by DPPH bio-autographic assay using thin layer chromatography (TLC) plate and 1, 1- iv diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging at different concentrations ranging from 0.2 to 10 mg/ml. The antibacterial activity was determined using disc diffusion method against Gram-positive and negative bacteria. The inhibition zones of HM and H. tiliaceus leaves fractions were measured.