Please use this identifier to cite or link to this item: http://umt-ir.umt.edu.my:8080/handle/123456789/15596
Title: Elucidation of the molecular mechanisms of acute phase protein gene expression regulated by interleukin-6 via peroxisome proliferated activated receptor a and CCAAT/enhancer binding proteins in human liver cells.
Authors: Muzaida Aminah Binti Mohd
Issue Date: Jun-2019
Publisher: Universiti Malaysia Terengganu
Abstract: The acute phase response (APR) is an orchestrated response to tissue injury and infection. A prominent feature of APR is the changes in the expression of liver’s proteins, known as acute phase proteins (APPs). It has been reported that IL-6 signalling regulate the activity of transcription factors including PPARα and C/EBPs which in turn, changes the pattern of APP gene expression. However, transcriptional regulation of APP by IL-6 via these transcription factors is not fully understood. Therefore, the aim of this study was to elucidate the molecular mechanisms of PPARα and C/EBP in mediating IL-6-regulated gene expression of APPs. The effects of transcription factors knockdown on the levels of APP mRNA displayed different pattern of changes upon PPARα and C/EBP knockdown with respective small interference RNA (siRNA). Therefore, the results indicated that PPARα and C/EBP members may have different mechanisms of action in modulating the effects of IL-6 on the gene expression of different APPs in HepG2 cells. This might be due to the diverse arrangements of transcription factor binding elements in different APP promoters as predicted using MatInspector.
URI: http://umt-ir.umt.edu.my:8080/handle/123456789/15596
Appears in Collections:Institut Bioteknologi Marin

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