Please use this identifier to cite or link to this item: http://umt-ir.umt.edu.my:8080/handle/123456789/10424
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dc.contributor.authorTeng Phei Yin-
dc.date.accessioned2018-11-21T07:51:36Z-
dc.date.available2018-11-21T07:51:36Z-
dc.date.issued2011-
dc.identifier.urihttp://umt-ir.umt.edu.my:8080/xmlui/handle/123456789/10424-
dc.description.abstractThe objectives of this study were to determine the effect of cryoprotectants and sperm density for a long-term storage of S. olivacea spermatozoa. Spermatozoa were obtained by homogenized the spermatophores by a glass homogenizer in an ice-bath and centrifugation at 4 ° C. Spermatozoa were suspended in calcium-free saline (Ca-F saline) containing the cryoprotectants glycerol, dimethyl sulfoxide (DMSO) and methanol at concentration of 5%. Sperm which were vibrated and rotated was counted as live in sperm viability assessment. Samples of spermatozoa were cooled to -196 ° C by two-step freezing, first to -80 ° C and then by plunging in liquid nitrogen (LN). Gradual cooling (1 ° C min- 1 ) was done by cooling the spermatozoa. Thawing was done at 30 ° C water bath for 2 min. This yielded live sperm after storage in LN for 30 days. The best sperm viability was obtained from density of 10 8 cells per ml in DMSO. There is no significant different (P > 0.05) between cryoprotectant toward sperm viability. However, there is a significant different (P < 0.05) between density toward the sperm viability.en_US
dc.language.isoenen_US
dc.publisherUniversiti Malaysia Terengganuen_US
dc.subjectTeng Phei Yinen_US
dc.subjectLP 46 FMSM 3 2011en_US
dc.titleEffect of different cyroprotectants for spermatozoa cyropreservation of orange mud crab, Scylla olivacea (Herbst, 1796)en_US
dc.typeWorking Paperen_US
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