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Title: | Electroporation of Chlorella sp. with p35s-AP linearized construct |
Authors: | Norbaiti Mohd Isa |
Keywords: | Norbaiti Mohd Isa LP 38 FST 2 2007 |
Issue Date: | 2007 |
Publisher: | Terengganu: Universiti Malaysia Terengganu |
Abstract: | Electroporation has been used in plants and microalgae genetic engmeenng to produce beneficial transgenic plants and microalgae. Genetic transformation by electroporation increase the level of polyunsaturated fatty acids in Ch/ore/la sp. such as m-3 C18:3 and m-6 C18:2. The objectives of this study are to transform Ch/ore/la sp. with p35S-AP construct by electroporatian and to select for the putative recombinant chlorella cells. The extracted plasmid DNA of p35S-AP was verified using PCR technique. In this step, three types of primer combination were used as follows: 35S-F/35S-R, PTE-VF1/PTE-VR2 and 35S-F/PTE-VF1. The 35S-F/35S-R primer combination successfully amplified the CaMV 35S promoter with the size of 326 bp. The combination of PTE-VF1/PTE-VR2 primer successfully amplified the antisense palmitoyl-ACP thioesterase gene fragment with the size of 617 bp while 35S-F/PTE-VF1 successfully amplified the CaMV 35S promoter and antisense palmitoyl-ACP thioesterase gene fragment with the size of 934 bp. The DNA plasmid was then digested with EcoRl restriction enzyme to produce linear plasmid followed by DNA plasmid purification. |
URI: | http://hdl.handle.net/123456789/8445 |
Appears in Collections: | Fakulti Sains dan Teknologi |
Files in This Item:
File | Description | Size | Format | |
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LP 38 FST 2 2007 Abstract.pdf | 814.7 kB | Adobe PDF | View/Open | |
LP 38 FST 2 2007 Full Text.pdf Restricted Access | 2.7 MB | Adobe PDF | View/Open Request a copy |
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