Please use this identifier to cite or link to this item: http://umt-ir.umt.edu.my:8080/handle/123456789/5461
Title: Potential Anti-Atherosclerotic Compound Isolated from Acanthaster planci
Authors: Nurul Hazirah Mat Lazim
Asnuzilawati Asari
Faridah Mohamad
Tg. Muhammad TS
Noraznawati Ismail
Aziz Ahmad
Mariam Taib
Habsah Mohamad
Keywords: Methyl benzoate
Acanthaster planci
SRB-1 promoter
anti-atherosclerotic agent
Issue Date: 2016
Publisher: Research Journal of Pharmaceutical, Biological and Chemical Sciences
Citation: Vol.7(1) ; 482-487 p.
Abstract: Methyl benzoate was isolated from the the outer layer of A. planci (APOL) and tested towards cytotoxicity to HepG2 cells and Luciferase assay. The compound was not toxic towards HepG2 cells with IC50 value of 150.71 ±11.31 μg/ml. Methyl benzoate was screened with PPRE promoter to determine its ability as PPAR ligand and expressed the target gene with maximum fold change of range 3.5 to 4.0 at concentration 0.78 μg/ml. The confirmation as PPARɤ ligand was carried out with SRB-1 promoter and the positive results were obtained. The compound showed optimum expression higher than positive control used at 3.12μg/ml with almost 5.0 fold change. In addition, expression of Luciferase genes was higher than positive control even at lower concentration 0.39 μg/ml. These results indicated that methyl benzoate from APOL have the potential as anti-atherosclerotic agent, might as well beneficial as pharmaceutical products to prevent atherosclerosis which should be further explored.
URI: http://hdl.handle.net/123456789/5461
Appears in Collections:Journal Articles

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